生长抑素诱导猕猴外周血中性粒细胞凋亡  

Effect of somstostatin on apoptosis of polymorphonuclear neutrophils of rhesus monkey

作　　者：许兰涛[1] 唐承薇[1] 

机构地区：[1]四川大学华西医院消化内科,四川省成都市610041

出　　处：《世界华人消化杂志》2005年第5期617-620,共4页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30170875~~

摘　　要：目的:观察生长抑素对猕猴外周血中性粒细胞的作用. 方法:用生长抑素与猕猴外周血中性粒细胞共同孵育一定时间后,采用流式细胞仪、DNA电泳、电镜观察猕猴外周血中性粒细胞的形态变化,细胞DNA含量分布变化. 结果:生长抑素能诱导猕猴外周血中性粒细胞凋亡, 在形态学上表现为细胞膜起泡、染色质固缩、核碎裂、凋亡小体形成:电泳呈凋亡特征性Ladder带;流式细胞仪分析显示,PMN凋亡比例增高(22.1%vs50.5%.P<0.01).AIM: To study the effect of somstostatin on the apoptosis of polymorphonuclear neutrophils (PMNs) in rhesus monkey. METHODS: PMNs were isolated from whole blood of normal rhesus monkey using dne-step discontinuous Percoll gradient centrifugation technique. PMNs were cultured with 5×10-5mol/L somstostatin. Cell morphology was examined by light and electronic microscopy. Apoptosis was detected by flow cytometry and DNA agarose electrophoresis. RESULTS: Somstostatin at 5×10-5mol/L induced significant apoptosis in PMNs. The cells manifested typical apoptotic morphology: cell membrane blebbing, chromatin condensation, nucleic fragmentation and apoptotic body formation. Agarose electrophoresis revealed specific DNA ladder. Flow cytometry analysis showed that the ratio of PMNs undergoing apoptosis was remarkably increased from 22.1 % to 50.5% following somstostatin treatment (P<0.01). CONCLUSION: Somstostatin induces apoptosis of PMNs in rhesus monkey. It may be a new target for the treatment of multiple organ dysfunction syndrome (MODS).

关 键 词：中性粒细胞凋亡 生长抑素 猕猴 外周血中性粒细胞 细胞DNA含量 流式细胞仪分析 DNA电泳 PMN凋亡 作用方法 形态变化 电镜观察 分布变化 细胞膜 形态学 染色质 核碎裂 特征性 

分 类 号：Q952.5[生物学—动物学]