白血病抑制因子LIF第29位氨基酸突变导致其功能完全丧失(英文)  

A LIF Mutation at The 29th Amino Acid Totally Abolished The Biological Functions

作　　者：宁红秀[1] 陈钺 张原江[1] 戎煜[1] 吴晓君[2] 张秀芳[1] 常智杰[1] 

机构地区：[1]清华大学生物科学与技术系 [2]北京大学第三医院,北京100083

出　　处：《生物化学与生物物理进展》2005年第4期318-324,共7页Progress In Biochemistry and Biophysics

基　　金：国家重点基础研究发展规划项目(973)(2001CB510006,2002CB51300);国家自然科学基金资助项目(39970369,30070703, 30470888和30030050)~~

摘　　要：白血病抑制因子(LIF)为一种多功能细胞因子,在生物发育及维持正常生理功能中发挥着重要的作用. LIF在所有的哺乳动物中都具有高度的保守性,某些氨基酸在不同物种中保持不变. LIF中29位氨基酸在所有的哺乳动物中都是Q. 为了研究Q对LIF功能的重要性,利用随机PCR的方法将29位氨基酸突变为R,并将突变后的LIF克隆到真核表达载体pcDNA6,在哺乳动物细胞中成功表达. 同时以克隆到的野生型LIF作为对照. 将分泌到细胞培养基中的野生型和突变LIF因子收集,通过EMSA实验以及荧光素酶报告系统检测,发现野生型LIF因子具有激活STAT3信号通路的生物学活性. 同时,3H-TdR掺入实验结果表明,野生型LIF因子能显著抑制鼠骨髓白血病细胞系M1的增殖。而29位氨基酸突变的LIF因子则完全丧失了以上功能,但所发现的突变没有显性负的作用. 结果充分说明,LIF中高度保守的29位氨基酸对LIF功能的维持具有重要的作用.Leukemia inhibitory factor (LIF) plays important roles in varieties of biological processes. This factor is highly conserved in mammalian animals and only one heterozygous LIF mutation was reported to cause the infertility of women. A LIF mutation was generated and the evidences were provided that the mutation of mature LIF at the 29th amino acid totally abolished its functions, including stimulation of STAT activation assayed by Luciferase reporter gene expression and EMSA experiments. In addition, the mutated LIF failed to inhibit the proliferation of M1 cells. The data indicated that the mutation of LIF did not have a dominant negative effect but lost the biological functions, suggesting that the 29th amino acid is critical for maintaining the activities of LIF.

关 键 词：白血病抑制因子(LIF) 突变 功能 细胞增殖 

分 类 号：Q7[生物学—分子生物学]