甄氏外瓶霉的核糖体基因分型研究  

Population studies of Exophiala jeanselmeis by sequence analysis in the ribosomal DNA

作　　者：李东明[1,3] 李若瑜[1,2] 王晓红[1,2] 万喆[1,2] 马圣清[1,2] 王端礼[1,2] 

机构地区：[1]中国科学院微生物研究所真菌地衣系统学重点实验室,北京100080 [2]北京大学真菌和真菌病研究中心,北京大学第一医院皮肤性病科 [3]北京大学第三医院皮肤科

出　　处：《北京大学学报（医学版）》2005年第2期167-171,共5页Journal of Peking University:Health Sciences

摘　　要：目的:应用核糖体基因(rDNA)序列对甄氏外瓶霉进行分型研究。方法:试验菌株包括甄氏外瓶霉及其变种 20株(模式株、标准株、临床及自然分离株),丛梗孢外瓶霉 8株,威尼克外瓶霉 1株 (标准株及临床和环境分离株),疣状瓶霉 1株。煮沸冷冻法提取DNA,常规PCR扩增核糖体基因及其转录间隔区,应用DNA多序列分析等方法进行研究。结果:甄氏外瓶霉核糖体基因及其转录间隔区变异较大,DNA序列分析显示,不同来源、不同致病性的甄氏外瓶霉可分为 7型,个别菌种与其他甄氏外瓶霉的遗传距离较大。结论:甄氏外瓶霉核糖体基因及其转录间隔区序列变异较大,个别菌株的归属有待于进一步研究。Objective: To study the populations of Exophiala jeanselmei strains from diverse sources using rDNA sequences. Methods: Tested strains included 20 isolates of Exophiala jeanselmei (type culture, reference culture, clinical and environmental isolates), 8 isolates of E. moniliae, 1 isolate of Exophiala werneckii, respectively. DNA was isolated with boiled-iced water method. Ribosomal DNA and its internal transcribed spacer regions were amplified and analyzed with sequencing. Results: The ribosomal genes of E. jeanselmei varied greatly. Twenty strains of this species were divided into 7 types. The genetic distances of some strains in this species were quite long. Conclusion: The ribosomal RNA genes and their internal transcript sequences vary more widely than those of the other species in this genus.

关 键 词：甄氏外瓶霉 核糖体基因 分型研究 DNA序列分析 PCR扩增 间隔区 遗传距离 序列变异 标准株 分离株 冷冻法 转录 致病性 步研究 菌株 临床 

分 类 号：R379.9[医药卫生—病原生物学] R383.24[医药卫生—基础医学]