电离辐射对离体培养O-2A祖细胞分化的影响  

Differentiation of Oligodendrocyte/type-2-astrocyte Progenitor Cells after Irradiation in vitro

作　　者：陈暑波[1] 田野[1] 夏春林[2] 万明辉[2] 

机构地区：[1]苏州大学附属二院肿瘤放疗科,江苏苏州215004 [2]苏州大学解剖学教研室,江苏苏州215007

出　　处：《中国神经肿瘤杂志》2005年第1期39-42,共4页Chinese Journal of Neuro-Oncology

基　　金：国家自然科学基金资助项目(No.30170287)江苏省卫生厅重大科研课题资助项目(No.K200406)

摘　　要：背景与目的:电离辐射引起中枢神经系统脱髓鞘病变的主要靶细胞之一为O-2A祖细胞,其在体外可定向诱导分化为少突胶质细胞和Ⅱ型星形胶质细胞。本文旨在探索电离辐射对O-2A祖细胞分化的影响,为研究早期放射性脑损伤发病机理的研究提供实验基础。方法:利用新生SD大鼠脑混合胶质细胞原代培养、定向诱导分化和免疫细胞化学等技术,观察不同辐射剂量对O-2A祖细胞分化的影响,检测其数量、形态和分化比例的情况。结果:倒置显微镜下计数对照组O-2A祖细胞个数为24.80±3.70,照射2 Gy、10 Gy、30 Gy组分别为4.40±0.89、1.60±0.55、1.20±0.45,与对照组相比照射后O-2A祖细胞总数明显减少(P<0.001); 10 Gy、30 Gy组中诱导分化的少突胶质细胞和Ⅱ型星形胶质细胞的突起变粗变短,但各组间细胞分化比例未见明显变化(P>0.05)。结论:电离辐射对O-2A祖细胞的分化有较大影响,影响程度与辐射剂量存在量效关系。BACKGROUND & OBJECTIVE: Oligodendrocyte/type-2-astrocyte progenitor cells (O-2A progenitors) is one of the target cells in radiation induced central nervous system injury. O-2A can be induced to differentiate into oligodendrocytes and type-2-astrocytes in vitro. In this study, we observed the effects of radiation on the differentiation of O-2A and provide basic data on the pathogenesis of brain radiation injury in early stage. METHODS: Differentiation of O-2A was observed after applying radiation in different dose to P0-P1 Sprague-Dawley Rat's mixed glial cells culture, and through induced differentiation as well as immunocytochemistry. After radiation, the quantities of O-2A, morphological changes and the ratio of the differentiation were measured. RESULTS: The number of O-2A was 24.80±3.70 in the placebo group. In 2Gy, 10Gy, 30 Gy group, the number was 4.40±0.89, 1.60±0.55, 1.20±0.45, respectively. After radiation, the quantities of O-2A reduced significantly (P< 0.001). Reduced short and thicker neuritis of oligodendrocytes and type-2-astrocytes were found in 10 Cy and 30 Cy group. There were no significant changes of the ratio of the differentiated oligodendrocytes and type-2-astrocytes among the groups (P >0.05). CONCLUSION: Dose dependent radiation may have determinate effect on the differentiation of O-2A.

关 键 词：细胞分化 电离辐射 离体培养 早期放射性脑损伤 星形胶质细胞 少突胶质细胞 定向诱导分化 中枢神经系统 细胞原代培养 免疫细胞化学 辐射剂量 脱髓鞘病变 祖细胞 发病机理 显微镜下 细胞总数 量效关系 影响程度 对照组 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R811.5[医药卫生—基础医学]