喘可治注射液抑制小鼠变应性接触性皮炎的实验研究  

Suppressive effect of Chuankezhi (CKZ) injection on murineal lergic contact dermatitis

作　　者：熊恺轩[1] 曾耀英[2] 蔡小嫦[1] 王通[2] 肇静娴[2] 易敏[1] 

机构地区：[1]暨南大学附属第一医院皮肤科,广东广州516032 [2]暨南大学组织移植与免疫教育部重点实验室,广东广州516032

出　　处：《中国病理生理杂志》2005年第4期757-760,共4页Chinese Journal of Pathophysiology

基　　金：国家"973"课题 (No .G19990 5 4 30 3) ;国家自然科学基金重点项目 (No .30 2 30 35 0 ;No .39930 2 30 ) ;广东省"十五"重大科技专项 (No.A30 2 0 2 0 2 0 4 )

摘　　要：目的:探讨中药喘可治注射液抑制小鼠变应性接触性皮炎(Ⅳ型变态反应)的效果。方法:将小鼠按给药不同分为喘可治注射液高、中,低剂量组,地塞米松(DEX)组,苯海拉明及生理盐水组,利用2 ,4 -二硝基氟苯诱发的小鼠左耳变应性接触性皮炎的动物模型,各组于第0d、1d致敏前2h ,第2d和第5d诱发前2h及诱发后6h腹腔注射给药,通过测量左耳厚度差、左耳重量差,体重差及观察左耳病理改变,比较不同剂量喘可治注射液之间以及与对照组之间的疗效。结果:喘可治注射液高、中、低剂量和DEX组小鼠左耳厚度及重量的增加,浸润的单核细胞和中性粒细胞的数量均明显低于生理盐水组(P <0 . 0 1) ;DEX组小鼠左耳红肿的程度虽稍低于喘可治组,但小鼠体重差与其它组比较均有显著差异(P <0 . 0 1) ;苯海拉明组小鼠变应性接触性皮炎的程度与生理盐水组差异无显著。结论:喘可治注射液具有明显抑制小鼠变应性接触性皮炎的作用。AIM: To study the suppressive effect of c hu ankezhi (CKZ) injection, a Chinese medicine, on murine allergic contact dermatit is (type IV hypersensitivity). METHODS: Mice were divided into 6 groups according to different medicine treatments: CKZ high, middle, low dose ( CKZⅠ,Ⅱ,Ⅲ) groups, dexamethasone(DEX), benadryl and saline groups. Murine alle rgic contact dermatitis was induced by intraperitoneal injection of 2, 4-dinitro fluorobenzene. Different medicines were administrated at 2 h before sensitizatio n on day 0 and day 1, day 2, 2 h before elicitation and 6 h after on day 5. The six experimental groups were compared according to left ear thickness difference (S1), left ear weight difference (S2), body weight difference (S3) and dermal i nflammatory infiltration cell number. RESULTS: Compared with saline group, the left ear swelling and d ermal inflammatory infiltration cell number were significantly reduced in CKZⅠ, Ⅱ,Ⅲ and DEX groups (P<0.01). The S1,S2 implied better effects of DEX trea tment, however, the body weight difference in DEX group was significantly less t han the other groups (P<0.01). The above parameters in benadryl group had n o significant difference with saline group. CONCLUSION: CKZ can significantly suppress murine allergic conta ct dermatitis.

关 键 词：超敏反应 迟发型 皮炎 变应性接触性 喘可治 

分 类 号：R758.22[医药卫生—皮肤病学与性病学]