喘可治延长小鼠异基因移植皮片存活时间与CD4^+CD25^+调节性T细胞相关性研究  

The relation between prolonged survival of skin allograft by chuan-ke-zhi and CD4^+CD25^+ regulatory Tcells

作　　者：王幼萍[1] 曾耀英[1] 肇静娴[1] 赵莉[1] 邢飞跃[1] 狄静芳[1] 江逊[1] 李海仙[1] 

机构地区：[1]暨南大学组织移植与免疫教育部重点实验室,广东广州510632

出　　处：《中国病理生理杂志》2005年第4期765-767,共3页Chinese Journal of Pathophysiology

基　　金：国家"973"项目 (No .G2 0 0 0 0 5 70 0 6 ) ;国家自然科学基金重点项目 (No.39930 2 30 )

摘　　要：目的:研究喘可治抑制小鼠异基因皮片移植排斥作用与小鼠体内CD4 + CD2 5 + 调节性T细胞(CD4 +CD2 5 + Tr)变化的相关性。方法:建立小鼠同种异基因与同基因皮片移植模型,通过腹腔注射给药喘可治(CKZ)观察其对皮片移植存活时间的影响,并利用3色免疫荧光标记和流式细胞术分析受鼠外周血CD4 + CD2 5 + Tr变化规律。结果:同种异基因移植CKZ组的移植皮片存活时间显著长于同种异基因移植对照组,分别为(19 .5±2 .3)d和(10 . 2±2 .2 )d ,P <0 . 0 1;在同种异基因皮片移植后,受体外周血CD4 + CD2 5 + Tr占CD4 + T细胞百分率明显升高,术后8d达到高峰(P <0 . 0 1) ,然后出现下降趋势,其中同种异基因移植对照组在术后13d时已回落至正常水平,而同种异基因移植CKZ组在术后2 3d时仍维持在高于移植前水平;在同基因皮片移植后,CKZ组与对照组外周血CD4 + CD2 5 +Tr水平均无明显升高(P >0 . 0 5 )。结论:喘可治可延长小鼠同种异基因移植皮片存活时间,通过升高CD4 + CD2 5 + Tr水平而发挥免疫抑制效应可能是其机制之一。AIM: To study the relation between prolon ge d survival of skin allograft by chuan-ke-zhi (CKZ, drug of Chinese herbal) and C D4+CD25+ regulatory T cells (CD4+CD25+ Tr) in mice. METHODS: Skin allograft and isograft model in mice were establi shed and CKZ was administered by intraperitoneal injection. To observe its influ ence on survival of the graft, three color fluorescent staining together with fl ow cytometry was used to analyze the change of CD4+CD25+ Tr. RESULTS: The survival of skin allograft in CKZ group was signifi cantly prolonged compared to control group, (19.5±2.3) days and (10.2±2.2) days, respectively, P<0.01. The ratio of CD4+CD25+ Tr to CD4+ T cell s in peripheral blood of the recipient went up after allogeneic transplantation, reaching peak at 8 d and then descended, which came back to normal level in cont rol group at 13 d but still higher than normal in CKZ group at 23 d. The CD4+C D25+ Tr level had no significant change in either CKZ group or control group a fter syngeneic transplantation (P>0.05). CONCLUSION: CKZ has an effect of prolonging the survival of skin allograft. Enhancement of CD4+CD25+ Tr might be one of the mechanisms under lying its immunosuppressive effect.

关 键 词：喘可治 CD4^+CD25^+调节性T细胞 移植 皮肤 流式细胞术 

分 类 号：R392.4[医药卫生—免疫学]