桥序列在构建人工核酶M1GS中的作用  

The role of bridge sequence in the construction of artificial ribozyme M1GS

作　　者：郑永霞[1] 李弘剑[1] 李月琴[1] 陈浩军[1] 唐冬生[1] 张欣[1] 周天鸿[1] 

机构地区：[1]暨南大学生物工程学系,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2005年第2期151-155,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家自然科学基金 (30 370 776 ) ;广东省自然科学基金重大项目 (36 70 3) ;广东省自然科学基金 (0 2 116 2 ;0 0 0 718)资助项目

摘　　要：目的:为检验连接的GS序列是否会影响M1RNA高级结构而导致M1RNA活性改变,对核酶的催化机制进行探讨。方法:通过软件模拟对M1GS进行结构分析,筛选了一段独立折叠的桥序列连接M1RNA与GS ,并通过体外切割实验检验有桥和无桥序列的核酶的活性。结果:有桥序列的M1GS具备体外特异切割底物的核酶活性,而无桥序列的M1GS核酶未表现体外切割活性。Aim: To elucidate the influence of covalently GS on the conformation and function of M1RNA in M1GS. Methods: Applying the RNA second structure software to simulate structure of M1GS, a sequence folded independently as bridge between M1RNA and GS was screen out. The enzyme activities of M1GS with bridge and M1GS without bridge were tested in vitro. Results: The M1GS with bridge can efficiently cleave the substrate, UL54 RNA fragment, however, the M1GS without bridge don't show activity of cleavage. Conclusion: It confirm that the bridge contribute to the completeness of confirmation of M1RNA, and play an important role in keeping the M1GS' activity of in vitro cleavage.

关 键 词：核酶 核酶P 人巨细胞病毒 UL54基因 

分 类 号：Q522[生物学—生物化学]