胎肝Sca-1^+细胞治疗STZ诱导小鼠糖尿病的实验研究  

Reversal of diabetes in the mice by injection of stem cell antigen positive cells from murine fetal liver

作　　者：林晨[1] 张洹[2] 廖继东[2] 赵欣[3] 姜铧[2] 余卫[2] 

机构地区：[1]暨南大学医学院微生物学与免疫学教研室,广东广州510632 [2]暨南大学医学院血液病研究所,广东广州510632 [3]暨南大学医学院中心实验室,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2005年第2期156-161,共6页Journal of Jinan University(Natural Science & Medicine Edition)

摘　　要：目的:探讨小鼠胎肝组织中的干细胞抗原1阳性的细胞(Sca - 1+细胞)治疗链脲佐菌素(STZ)诱导糖尿病鼠的潜能。方法:取14 5d的C5 7BL/6J小鼠胎肝,制作细胞悬液,用单克隆免疫磁珠细胞分离技术分离Sca - 1+细胞,将2×10 5个雄性小鼠Sca - 1+细胞输注到STZ诱导的C5 7BL/6J雌性小鼠体内,以后每7d定时测定小鼠血糖,第38d处死受体小鼠取胰腺组织固定、切片,免疫组化观察胰腺组织中胰岛素阳性的β细胞变化。结果:小鼠胎肝Sca 1+细胞能够有效抑制STZ诱导小鼠血糖的持续升高,明显降低糖尿病鼠的死亡率。受体小鼠胰岛细胞结构清楚,其中可见表达胰岛素的β细胞,荧光原位杂交显示小鼠胰岛内有Y染色体阳性杂交点。结论:小鼠胎肝Sca -Aim: To explore the potential of stem cell antigen 1 positive cells (Sca-1^+ cells) from murine fetal liver to treat STZ-induced diabetes in mice. Methods: The (Sca-1^+) cells from the livers of C57BL/6J male murine fetuses aged 14.5 days were separated with the Magnetic cell sorting(MACS) technology. 2×10~5 Sca-1^+ cells from the male murine fetuses were transplanted through the caudal vein into STZ-induced female mice of same line aged (8-12) weeks. Blood glucose were measured weekly from day 0 to day 38. The pancreas of each mouse was taked out and fixed in 10% buffered formalin at day 38. Results: Sca-1^+ cells can inhibit increasing of blood glucose and reduce the mortality of STZ-induced diabetes in mice remarkablely compared with control mice. The size of islet in pancreas in recipient mice with Sca-1^+ cells treatment showed larger and the number of islet cell increased. Sca-1^+ cells of liver donor male differentiate into islet within the pancreas showed by FISH method. Conclusion: Sca-1^+ cells from murine fetal liver could play potentially role in treatment of STZ-induced diabetes.

关 键 词：干细胞 链脲佐菌素 糖尿病 

分 类 号：R979.5[医药卫生—药品]