用抑菌圈-定殖力双重测定法筛选姜瘟病的生防细菌研究  

Screen of Biocontrol Bacteria of Ginger Wilt by Inhibiting Zones and Root-colonizing Capacity

作　　者：刘铭[1] 张敏[1] 尹福强[1] 涂勇[1] 张宇[1] 戢俊臣[1] 

机构地区：[1]四川农业大学农学院,四川雅安625014

出　　处：《中国农学通报》2005年第4期266-269,共4页Chinese Agricultural Science Bulletin

基　　金：四川省应用基础项目研究资助(项目编号04JY029-029)

摘　　要：2003年6月在雅安飞仙关多功乡13个未发病或发病较轻的生姜田块中分离到217个细菌菌株,其中三个(L1、D4、T10)菌株对姜瘟病有强抑菌作用,用利福平标记后在室内的灭菌土和非灭菌土中分别进行定殖能力的测定,结果表明:L1在平板上的抑菌圈不是最大,而最终定殖密度却是最大,而T10抑菌圈最大,定殖能力却不是最大。在灭菌土中比非灭菌土中的定殖能力强。在田间防病试验中,防病效果分别达60.16%(L1)、39.10%(D4)、52.39%(T10)、此外试验结果还表明三个菌株都有不同程度的增产促长作用。Among 217 bacteria strains isolated from 13 disease-free and lower field soil of ginger in Fei Xian-guan, Yaan, L1,D4, T10 had effective antagonism against ginger wilt. Colonizing capacity was conducted by label Rif in autoclaved and non-autoclaved soil. The results showed that inhibiting zones of L1 wasn' t largest on the plates, while its colonizing dense was largest. Inhibiting zones of T10 was largest, while colonizing capacity wasn' t largest. Colonizing capacity was higher in autoclaved soil than that in non-autoclaved soil. In this field disease controlling trial, disease-controlling effects of L1,D4, and T10 reached 60.16%, 39.10%, 52.39%, respectively. It also showed that the three strains had functions of increasing yield and growth in different levels.

关 键 词：抑菌圈 姜瘟病 生防细菌 测定法 定殖力 2003年6月 筛选 定殖能力 抑菌作用 防病试验 防病效果 促长作用 试验结果 灭菌 菌株 利福平 T10 发病 田块 生姜 田间 

分 类 号：S896.6[农业科学—特种经济动物饲养] S436.32[农业科学—畜牧兽医]