硫酸卡那霉素体外抑制呼吸道合胞病毒作用的研究  

Anti-respiratory syncytial virus activity of kanamycin sulfate in vitro

作　　者：方学平[1] 梅兴国[1] 龚伟[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《中国抗生素杂志》2005年第4期247-249,共3页Chinese Journal of Antibiotics

摘　　要：目的评价硫酸卡那霉素体外抗呼吸道合胞病毒作用机制。方法采用细胞培养技术观察硫酸卡那霉素对细胞的毒性和抗呼吸道合胞病毒作用。结果用空斑减数实验测得半数有效剂量(EC50)为(1.71±0.23)mg/ml,MTT法测定其半数中毒浓度(CC50)为(12.36±0.85)mg/ml,SI为7.2,SI>4(有意义);在不同时间给药对病毒的抑制实验中,呼吸道合胞病毒感染喉癌上皮细胞(Hep-2)细胞后1、2、4、6、8和10h给药均有抑制作用(P<0.05),穿入和吸附抑制实验表明其对病毒穿入过程有明显地抑制作用(P<0.05),但是对吸附过程没有抑制作用。硫酸卡那霉素对RSV病毒没有直接的灭活作用。结论硫酸卡那霉素在体外实验中对呼吸道合胞病毒穿入细胞及在细胞内复制的过程均有抑制作用。Objective To evaluate the mode of inhibition of respiratory syncytial virus by kanamycin sulfate. Methods The cytotoxicity and antiviral activity against respiratory syncytial virus (RSV) in cell culture were tested. Results The median effective concentration (EC_ 50 ) of kanamycin sulfate against replication of the Long strain of RSV in HEp-2 cells was (1.71±0.23)mg/ml by the plaque reduction method. The median cytotoxicity concentration (50% cytotoxicity concentration, CC_ 50 ) of the kanamycin sulfate was (12.36±0.85)mg/ml by 3-[(4,5-dimethylthiazole-2-yl) 2,5-diphenyl tetrazolium bromide] MTT assay. The selectivity index [SI](CC_ 50 for HEp-2: EC_ 50 ) of kanamycin sulfate for RSV was 7.2. SI>4 is considered significant. Moreover, kanmycin sulfate gave a time-course experiments in inhibiting RSV. In this assay testing the effect of kanamycin sulfate on virual growth, it showed inhibitory activity on RSV when it was added at 1h, 2h, 4h, 6h, 8h and 10h after infection ( P <0.05). By an inhibition assay for virus adsorption and penetration during the period of contact between the virus and cells, kanamycin sulfate inhibited RSV penetration into the cells ( P <0.05) but it didn′t inhibit RSV adsorption on the cell. Kanamycin sulfate didn′t show direct inactivatied effect on RSV. Conclusion kanamycin sulfate inhibited RSV penetration into the cells and replication in vitro .

关 键 词：硫酸卡那霉素 呼吸道合胞病毒 抗病毒作用 

分 类 号：R965[医药卫生—药理学]