猪孕酮酶免疫分析及释放激素对猪孕酮含量的影响  

The Enzymeimmunoassay of swine progesterone and effect of Iuteinizing hormone-releasing hor-mone on progesterone concentration in sows

作　　者：黄利权[1] 蔡荣湘[1] 徐士清[1] 孙仁华[1] 

机构地区：[1]浙江农业大学动物科学学院,浙江农业科学院

出　　处：《浙江农业大学学报》1994年第5期525-529,共5页

基　　金：浙江省科委资助

摘　　要：应用孕酮单克隆抗体（ＰＭｃＡｂ）和孕酮－辣根过氧化物酶（Ｐ－ＨＲＰ）通过微量板对猪血清孕酮进行酶免疫分析。结果表明：１：２．５×１０￣４浓度的ＰＭｃＡｂ和１：５×１０￣４浓度的Ｐ－ＨＲＰ之间具有较强的亲和力，母猪在发情配种前注射释放激素类似物（ＬＲＨ－Ａ＿３）能提高怀孕早期的孕酮含量，怀孕母猪在怀孕１５天时的孕酮含量是未孕猪的近２倍，分别为１７．２４ｎｇ／ｍＬ和９．５０８ｎｇ／ｍＬ。An enzymeimmunoassay of swine progesterone was developed using progesterone monoclone antibody(PMcAb and progesterone-horseradish peroxidase (p-HRP)in the microtitreplates. There was a stronger affinity between (PMcAb ) and P-HRP when the concentrations were1:2.5× 10￣4 and 1 :5× 10￣4, respectively.An increasing progesterone concentration of serum in ear-ly pregnancy was found in the sows to whom luteinizing hormone analogue(LRH -A_3)had been ad-ministered before in semination in comparison with control animals,On the 15th day of pregnancy,progesterone concentration in pregnant sows was 17.24 ng/mL being 2 times that in non-pregnant sows(9. 58 ng/mL).

关 键 词：酶免疫分析 孕酮 释放激素 母猪 

分 类 号：S828.3[农业科学—畜牧学]