甘丙肽及其受体在嗅成鞘细胞中的表达及对细胞增殖的影响  

THE EXPRESSION OF GALANIN AND GALANIN RECEPTORS IN OLFACTORY ENSHEATHING CELLS AND EFFECTS OF GALANIN ON CELLS PROLIFERATION

作　　者：夏趁意[1] 戚艳婷[1] 张烨[1] 袁崇刚[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062

出　　处：《实验生物学报》2005年第2期98-104,共7页Acta Biologiae Experimentalis Sinica

摘　　要：本实验从新生大鼠嗅球中分离出嗅成鞘细胞,进行体外培养。运用RT-PCR方法检测甘丙肽及其受体在体外培养的嗅成鞘细胞中的表达;运用MTT法检测甘丙肽及其受体激动剂、拮抗剂对嗅成鞘细胞增殖的影响。结果显示:嗅成鞘细胞表达甘丙肽(GAL)及其受体GalR2,而不表达其他两种受体GalR1和GalR3;甘西肽及两种受体激动剂GAL1-11和GAL2-11能够明显地抑制体外培养的嗅成鞘细胞的增殖,这一效应可被非特异性甘丙肽受体拮抗剂M35所阻断。Olfactory ensheathing cells (OECs) were isolated from newborn rat olfactory bulb and cultured in vitro. RT-PCR was used to determine the expression of galanin and it's receptors in these cells. MTT analysis was used to detect the effects of galanin and agonist, antagonist of galanin receptors on the proliferation of OECs. Results show that OECs express mRNAs for galanin and GalR2 but not for two another receptors, GalR1 and GalR3. In addition, galanin and two receptor agonists, GAL1-11 and GAL2-11, can significantly inhibit the proliferation of OECs. But the influence can be blocked by M35, nonspecific antagonist of galanin receptors.

关 键 词：细胞增殖 甘丙肽 受体激动剂 体外培养 PCR方法 受体拮抗剂 新生大鼠 MTT法 细胞表达 非特异性 检测 嗅球 

分 类 号：Q26[生物学—细胞生物学]