小麦-中间偃麦草双体异附加系的选育和鉴定  

CULTIVATION AND IDENTIFICATION OF WHEAT-ELYTRIGIA INTERMEDIUM ALIEN DISOMIC ADDITION LINES

作　　者：赵逢涛[1] 王黎明[1] 李文才[1] 林小虎[1] 李兴锋[1] 高居荣[1] 王洪刚[1] 

机构地区：[1]山东农业大学农学院国家小麦改良中心泰安分中心,泰安271018

出　　处：《实验生物学报》2005年第2期133-140,共8页Acta Biologiae Experimentalis Sinica

基　　金：国家自然科学基金(39970458)

摘　　要：在小麦-中间偃麦草59个杂交后代种质系中,筛选出6个小麦-中间偃麦草双体异附加系(line0605,line0607,line0609,line0610,line0611,line0625),并对其进行了形态学、白粉病抗性、细胞学和RAPD鉴定。形态学结果表明:6个双体异附加系农艺性状较好地结合了双亲的优良特点;细胞学结果表明:6个双体异附加系具有高度的细胞学稳定性,花粉母细胞减数分裂中期Ⅰ(PMCMI)的染色体构型为2n=22Ⅱ;RAPD分析表明:在供试的209个随机引物中有5个引物分别能在6个异附加系中稳定地扩增出不同的特异带型,可以作为各个异附加系所附加染色体的特异分子标记;白粉病抗性鉴定结果表明:line0605表现免疫,line0610和line0625表现高抗,line0607表现中抗,line0609和line0611表现中感。6 alien disomic addition lines (line0605, line0607, line0609, line0610, line0611, line0625), which were selected from 59 wheat-Elytrigia intermedium, hybridization germ plasm lines, were identified by using the methods of morphology, powdery mildew resistance identifica- tions, cytology and RAPD analysis. Morphogy results showed that they all had the better agronom- ic characters of their parents; Cytogic results showed that the chromosome configuration at PMC M Ⅰ was 2n=22 Ⅱ; 209 random primers were used for RAPD amplification, result showed 5 of them amplified the specific bands of Elytrigia intermedium parents of 6 disomic addition lines, which could be used as RAPD markers for alien addition chromosomes. Results of powdery mildew resistance showed that line0605 was immune, line0607 was intermediately resistant, line 0610 and line0625 were highly resistant, and line0609 and line0611 were intermediately sensitive.

关 键 词：双体异附加系 中间偃麦草 小麦 line 白粉病抗性 选育 RAPD鉴定 染色体构型 花粉母细胞 附加染色体 细胞学 杂交后代 农艺性状 减数分裂 随机引物 分子标记 鉴定结果 形态学 种质系 稳定性 分析表 免疫 

分 类 号：S512.1[农业科学—作物学] TP334.7[自动化与计算机技术—计算机系统结构]