红毛五加多糖的分离纯化和表观分子质量测定  

Isolation and Molecular Weight of Polysacharides from Acanthopanax girialdii Hams

作　　者：陈永[1] 卢戌[1] 谭晓晶[1] 孟尧[1] 辛渝[1] 许晓燕[1] 杨波[1] 李强[1] 于彩霞[1] 孟延发[1] 

机构地区：[1]四川大学生命科学学院,四川成都610041

出　　处：《四川大学学报（自然科学版）》2005年第2期373-376,共4页Journal of Sichuan University(Natural Science Edition)

摘　　要：红毛五加经热水浸提,用Sevag法脱蛋白,经DEAE cellulose32离子交换层析和SephacrylS 200HR凝胶层析纯化得到3个主要多糖组分:AHP I、AHP II、AHP Ⅲ.经HPLC和SephacrylS 300HR凝胶层析鉴定,3种多糖均为均一组分.HPLC测定AHP I、AHP II、AHP Ⅲ的表观分子质量分别为:7.0kD、59.5kD和12.9kD,SephacrylS 300HR凝胶层析进行验证,得到相似结果.Three water-solube polysaccharides, AHP-Ⅰ, AHP-Ⅱ and AHP-Ⅲ from the cortices of Acanthopanax giraldii Hams, were isolated and purified by ion-exchange chromatographies on DEAE-cellulose 32 and Sephacryl S-200.Three fractions were shown to be homogeneous by Sephacryl S-300,and HPLC. The apparent average molecular weight of AHP-I, AHP-II and AHP-Ⅲ was estimated to be approximately 7.0 kD,(59.5 kD )and 12.9 kD respectively by HPLC.

关 键 词：红毛五加 多糖 分离纯化 

分 类 号：Q539[生物学—生物化学]