儿茶提取物抗甲型流感病毒作用的实验研究  

Effect of Chinese Herb-catechu Extract on Influenza A Virus

作　　者：赵文明[1] 郑群[1] 刘振龙[1] 平国玲[1] 丁丽新[2] 石伟先[2] 刘海林[2] 

机构地区：[1]首都医科大学微生物学教研室 [2]北京市疾病预防控制中心

出　　处：《首都医科大学学报》2005年第2期167-170,共4页Journal of Capital Medical University

摘　　要：目的　研究从中药儿茶中提取的药物成分抗甲型流感病毒的作用。方法　用狗肾传代MDCK细胞培养法观察儿茶提取物抑制甲型流感病毒的致细胞病变作用;用鸡胚培养法观察儿茶提取物抑制甲型流感病毒增生的作用;用红细胞凝集试验观察儿茶提取物体外直接抑制甲型流感病毒的作用。结果　1)儿茶提取物的细胞毒性较低,可有效抑制甲型流感病毒感染细胞(P<0.05);2)儿茶提取物在一定范围内可明显抑制甲型流感病毒在鸡胚内的增生,经红细胞凝集试验测定抑制病毒效价为8倍或8倍以上;3)儿茶提取物直接与甲型流感病毒作用后可抑制病毒血凝效价达16 倍以上。结论　儿茶提取物具有很好的抗甲型流感病毒的作用。Objective To investigate the inhibitory effect of catechu extract to influenza A virus. Methods The inhibitory cytopathic effects (CPE) of catechu extract on influenza A virus were observed by MDCK cell culture techniques. The inhibiting influenza A virus effect of catechu extract was observed by means of chick-embryo culture, and the inhibitory activity of catechu extract on influenza A virus in vitro was observed by the hemagglutination inhibition test. Results 1) Cytotoxicity of catechu extract was lower and the catechu extract could inhibit influenza A virus from infecting MDCK significantly. 2) The catechu extract showed significant inhibition on the influenza A virus in the groups within the limits of dose on chick-embryo culture and the hemagglutination inhibition titer was over 8 times higher than the control group. 3) The catechu extract had distinctly inhibitory functions on influenza A virus, and the hemagglutination inhibition titer was over 16 times higher than the control group. Conclusion The chinese herb-catechu extract has strong effect on anti-influenza A virus.

关 键 词：儿茶提取物 病毒作用 实验研究 抗甲型流感病毒 致细胞病变作用 红细胞凝集试验 病毒感染细胞 抑制病毒 细胞培养法 药物成分 MDCK 试验观察 细胞毒性 血凝效价 增生 鸡胚 

分 类 号：R373.13[医药卫生—病原生物学] R563.1[医药卫生—基础医学]