成年SD大鼠心肌细胞的培养  

Isolation and culture of adult SD rat cardiomyocytes

作　　者：翟迎九[1] 董雪红[1] 周丽诺[1] 胡仁明[1] 

机构地区：[1]复旦大学医学院附属华山医院内分泌科,内分泌糖尿病研究所,上海200040

出　　处：《第四军医大学学报》2005年第8期705-707,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30270627)

摘　　要：目的: 建立稳定的成年SD大鼠心肌细胞分离和培养的方法.方法: 主动脉插管逆行灌流分离成年SD大鼠心肌细胞,进行体外培养,并观察其形态的变化.结果: 刚分离的心肌细胞呈长杆状,表面有明显横纹,非同步博动,在含血清的培养环境中,细胞形态很快发生变化,出现去分化表现,在无血清的培养环境中,细胞保持刚分离时的在体形状, 2wk后细胞均明显萎缩.结论: 主动脉插管逆行灌流的方法是较为理想的成年SD大鼠心肌细胞分离与培养方法.AIM: To establish a stable method to isolate and culture adult SD rat cardiomyocytes.METHODS: The adult SD rat cardiomyocytes were isolated by perfusing retrogradely via the aorta and then cultured in vitro.The morphological changes of cardiomyocytes were observed.RESULTS: Freshly isolated cells were rod-shaped and striated,beating asynchronously.The cells cultured under the condition with fetal calf serum(FCS)changed rapidly in morphology and presented dedifferentiation,while those cultured under the condition without FCS remained unchanged,and the cells still rod-shaped and striated.After two weeks,the two kinds of cells all atrophied.CONCLUSION: Perfusing retrogradely via the aorta is a successful and ideal method to isolate and culture adult SD rat cardiomyocytes.

关 键 词：大鼠 心肌/细胞学 逆行灌流 细胞 培养的 

分 类 号：Q813.11[生物学—生物工程]