野蚕染色标记方法研究  

A study ou the stain 一markiny method for wild silkworms

作　　者：顾伟平[1] 夏大荣[1] 杨荣新[1] 张严峻[1] 

机构地区：[1]浙江省农业科学院原子能利用研究所

出　　处：《浙江农业学报》1994年第4期267-269,共3页Acta Agriculturae Zhejiangensis

摘　　要：本文论述了用碱性紫５ＢＮ液对野蚕进行体外染色标记的简便方法，试验表明，野蚕成虫经乙醚麻醉３一７ｍｉｎ后，用碱性紫５ＢＮ液喷雾染色标记，其产卵量，孵化率和寿命均与未标记成虫无明显差异。染色标记后的成虫在糖醋液，皂液和清水等各种诱捕液中浸泡１２ｈ后，其分辨率均达到１００％，浸泡２４ｈ后，其分辨率达到９０％左右，标记后的成虫能与非标记成虫明显区别，可用于测定辐射不育野蚕田间扩散能力。This paper presents a easy method of marking wild silkworms extertially with alkaline purple 5BN solution,The experiment Showed.the adult wild silkworms,after 3-7minutes' anesthesia in diethyl ether and then sprayed alkaline purple 5BN solution on had no significant difference with the untreated ones in their oviposition,egg hatchability and lifespan. As the marked silkworms were soaked for l2 hours in attractants such as the mixture of sugar and Vinegar solution,soap solution and fresh water. the differentiation rate was 100%,it soaked for 24 hours,merate was about 90%. It indicated hat the marked silkworms could be easily distinguished from the untreated ones This sufe method can be applied to ecological studies such as the assay of the irradiated sterile wild silkworm' spread ability in fields and behavior of the adult wild silk worms etc.

关 键 词：蚕 野蚕 染色标记 

分 类 号：S885.9[农业科学—特种经济动物饲养]