+Gz暴露后大鼠海马生长抑素与学习能力的变化  

Changes of Hippocampus Somatostatin and Learning Ability in Rats after+Gz Exposure

作　　者：曹新生[1] 孙喜庆[1] 吴燕红[1] 吴兴裕 刘挺松[1] 张舒[1] 

机构地区：[1]第四军医大学航空航天生物动力学教研室，陕西西安710032

出　　处：《航天医学与医学工程》2005年第2期79-83,共5页Space Medicine & Medical Engineering

基　　金：Foundationitem:supportedbytheNationalNaturalScience Foundation(39800158)

摘　　要：目的探讨持续性正加速度(+Gz)暴露后,缺血恢复期大鼠学习能力和海马生长抑素的变化规律。方法雄性SD大鼠80只随机分为对照组、+6Gz/3min组和+10Gz/3min组,观察不同G值+Gz作用后,0d、2d、4d和6d大鼠(每组8只)学习能力的变化,及+Gz暴露后0d、2d和4d大鼠海马生长抑素的变化情况(每时间点每组8只)。结果Y型迷宫测试中,与对照组比较,+6Gz/3min组正确反应次数和反应时在0d无显著性改变,在暴露后2d、4d和6d时正确反应次数均较对照组显著减少(P<0.01),反应时均显著延长(P<0.01);+10Gz/3min组在暴露后各时间点的正确反应次数均显著减少(P<0.01),反应时均显著延长(P<0.01);与+6Gz/3min组比较,+10Gz/3min组的正确数仅在0d时显著减少(P<0.01),反应时显著延长(P<0.05),余无显著差异。放射免疫结果显示,与对照组比较,+6Gz/3min组在暴露后0d,2d海马SS显著降低(P<0.05或P<0.01);+10Gz/3min组在暴露后0d,2d时间点海马SS显著降低(P<0.01)。结论+Gz暴露导致大鼠学习能力受损,海马生长抑素含量降低。Objective To investigate changes of learning ability and somatostatin (SS) changes after positive acceleration (+Gz) exposures. Mehtod Eighty male SD rats were randomly divided into 3 groups: control group(Con), +6 Gz/3 min group (+6 Gz), and +10 Gz/3 min group (+10 Gz),8 rats in each group. Changes of learning ability in rats were observed at 0 d, 2 d, 4 d and 6 d after +Gz exposure. SS in hippocampus was measured by RIA at 0 d, 2 d and 4 d after +Gz exposures ( there were 8 rats every time, in each group). Result In Y-maze test,number of correct response decreased significantly (P<0.01),and total reaction time increased significantly(P<0.01) in +6 Gz and +10 Gz groups as compared with control group; number of correct response and total reaction time in +10 Gz group changed significantly at 0 d(P<0.01 or P<0.05) as compared with +6 Gz group. RIA showed that, content of SS in hippocampus declined at 0 d and 2 d(P<0.05 or P<0.01)in +6 Gz and +10 Gz groups as compared with control group. Conclusion +Gz exposure could impair learning ability of rats, and inhibit expression of SS in hippocampus.

关 键 词：正加速度 +GZ 海马 学习能力 生长抑素 

分 类 号：R852.21[医药卫生—航空、航天与航海医学]