泰乐菌素产生菌的空间诱变育种研究  

A Study on Space Mutation of Streplomyces Fradiae.

作　　者：方晓梅 赵志甲 顾海科 

机构地区：[1]东方红宇航技术有限公司,北京100086 [2]西安亨通光华制药有限公司,陕西西安710075

出　　处：《航天医学与医学工程》2005年第2期121-125,共5页Space Medicine & Medical Engineering

基　　金：国家863计划(2002AA744063)资助

摘　　要：目的研究弗氏链霉菌(streptomycesfradiae)在空间条件下的变异规律,并筛选高产菌株应用于工业生产。方法将弗氏链霉菌(streptomycesfradiae)9940S+86连续经“神舟”1、3、4号飞船搭载进行太空诱变育种,统计筛选结果,初步探讨泰乐菌素产生菌在空间条件下的变异规律。结果复筛后得到48株效价较出发菌株提高20%以上的菌株,其中总效价最高达到14950μg/ml(摇瓶),较出发菌株提高91.5%。结论弗氏链霉菌的空间诱变受飞行时间影响较大。菌株经多次搭载后其产量变异有累积作用,并且产量变异和形态变异存在相关性。通过中试、生产实验,最终选定产量较出发生产菌株提高18%的T11568423菌株投入试生产。Objective To study the rule of mutation of streptomyces fradiae during spaceflight, and to select efficient tylosin producing strains for industrial production. Method Streptomyces fradiae 9940S +-86 were carried on-board spaceship “Shenzhou” Ⅰ,“Shenzhou” Ⅲ and “Shenzhou” Ⅳ sequentially to achieve spaceflight mutation breeding experiment. Result After space experiments and the screening tests in the lab, 48 strains were obtained which promoted production by +20% or more at shaker level. And the highest production of a strain was 14 950 μg/ml, which means an increase of 91.5%. Conclusion Comparing the results of three tests, it is found that the outer space environment can lead to a cumulative mutation. After the medium scale tests and production experiments, strain T1-156-84-23 was finally selected to be used for sample production.And its output was increased by 18%.

关 键 词：空间诱变 泰乐菌素 弗氏链霉菌 菌株 筛选 

分 类 号：Q693[生物学—生物物理学]