核酶阻断per1表达对吗啡成瘾小鼠海马c-fos转录及表达的影响  

The Effect of Ribozyme Specially Cleaving per1 mRNA on c-fos mRNA and Its Expression in Hippocampus of Morphine Addicted Mice.

作　　者：刘英辉[1] 彭涛[2] 陈立国[3] 肖静[2] 王正荣[2] 刘延友[2] 

机构地区：[1]四川大学基础与法医学院生物化学与分子生物学教研室,四川成都610041 [2]四川大学基础与法医学院生物医学工程研究室,四川成都610041 [3]天津市儿童医院检验科,天津300074

出　　处：《航天医学与医学工程》2005年第2期151-153,共3页Space Medicine & Medical Engineering

基　　金：国家自然科学基金资助(39970275;30070278)

摘　　要：目的研究用特异性核酶阻断生物节律基因per1的表达对吗啡成瘾小鼠海马c-fos基因转录及表达的影响。方法制备吗啡成瘾小鼠模型,向小鼠脑室内注射脂质体包裹的重组质粒pcDNA3.1—per1RZDNA,在脑内产生特异性核酶-per1RZ,阻断小鼠脑内生物节律基因per1的表达。作脑组织冰冻切片,用原位杂交和免疫组化法测定小鼠海马c-fos基因转录及表达的变化。结果per1RZ能使吗啡成瘾小鼠海马的c-fos基因转录及表达明显减弱。结论重组质粒表达的per1RZ可以干扰生物节律基因per1的表达,抑制海马c-fos基因的转录和表达,从而控制吗啡成瘾的发生。Objective To study the change of c-fos mRNA and protein in hippocampus of morphine addicted mice after injected with ribozyme specially cleaving per1 mRNA. Method The recombined plasmid pcDNA 3.1—per1RZ DNA was injected into the ventricles of morphine addited mice to transcript the corresponding ribozyme which cleaves per1 mRNA particularly. And then, the brains of mice were fixed by perfusion. The level of c-fos mRNA was assayed by in situ hybridization and c-fos protein was detected by immunohistochemical staining. Result The level of c-fos mRNA and protein decreased after injection of the recombined plasmid pcDNA 3.1—per1RZ DNA expressing the ribozyme cleaving per1 mRNA. Conclusion The ribozyme specially cleaving per1 mRNA has potential function in inhibiting the transcription and expression of c-fos and blocking the morphine addition.

关 键 词：基因控制 基因表达 核酶 节律基因 c—fos PER1 吗啡 成瘾 

分 类 号：Q756[生物学—分子生物学]