植物病原细菌中超氧阴离子释放及其释放位点的研究  

Production and releasing sites of superoxide anion in phytopathogenic bacteria

作　　者：李欣[1] 李红玉[1] 庞新跃[1] 王金生[2] 李敏权[3] 段建功[1] 杨福军[4] 

机构地区：[1]兰州大学生命科学学院,兰州730000 [2]南京农业大学农业部病虫监测与治理重点实验室,南京210095 [3]甘肃农业大学植物保护学系,兰州730060 [4]兰州生物制品研究所,兰州730060

出　　处：《微生物学报》2005年第2期283-287,共5页Acta Microbiologica Sinica

基　　金：国家自然科学基金 (3 0 170 2 3 8)~~

摘　　要：实验发现很多植物病原细菌具有自身释放超氧阴离子的现象 ,其释放规律与菌株的致病性可能存在一定的关系。并且植物病原细菌超氧阴离子的释放是多位点的 ,在细胞膜、细胞壁及无菌滤液中用化学方法及电子自旋共振法 (Electronspinresonance ,ESR)都能够检测到超氧阴离子的释放。无论是自然生理状态下 ,还是SOD酶活性被抑制后 ,都显示各组分中无菌滤液的超氧阴离子浓度最高 ,可能是植物病原细菌超氧阴离子释放的主要位点。Many phytopathogenic bacteria were founded to release the superoxide anion (O- 2) by themselves from different sites and the production of O- 2 was related to the pathogenicity of strains. The presences of O- 2 in cytomembrane, cell wall and filtrate were confirmed by chemical method and electron spin resonance (ESR). The results show that filtrate may be the primary site of O- 2 production due to its higher O- 2 generating activity either in the presence or the absence of DDC. Additional lines of evidence further suggested a regulatory mechanism of O- 2 production in phytopathogenic bacteria.

关 键 词：植物病原细菌 超氧阴离子释放 电子自旋共振(ESR) 

分 类 号：S432.4[农业科学—植物病理学]