马铃薯及番茄晚疫病菌的核糖体DNA-ITS区段序列分析  

Analysis on Sequence of Ribosomal DNA-ITS of Phytophthora infestans from Potato and Tomato

作　　者：杨雅云[1] 罗文富[1] 杨艳丽[1] 

机构地区：[1]云南农业大学植物保护学院,云南昆明650201

出　　处：《云南农业大学学报》2005年第2期188-192,共5页Journal of Yunnan Agricultural University

基　　金：云南省自然科学基金项目(97C023M);云南省科技攻关项目(2002NG09)

摘　　要：取3个不侵染马铃薯的番茄晚疫病菌菌株和3个不侵染番茄的马铃薯晚疫病菌菌株,以及2个可交叉侵染马铃薯和番茄的菌株,提取DNA全基因组后,用引物ITS1和ITS4进行扩增,得到约850bp左右的扩增产物并进行测序,比较同源性,结果显示这些菌株的全基因组DNA序列与Genebank上登录的3个序列AF228084,AF228083,AY269995 1的同源性均为100%。3个番茄菌株之间的同源性大小为98 22%,3个马铃薯菌株之间的为99 52%,较番茄菌株的同源性高。不可交叉的两类菌株间的同源性为98 28%,可交叉侵染的番茄菌株和马铃薯菌株之间的同源性为99 17%,比不可交叉侵染的菌株间的同源性高,说明相似的序列使得生物学特性也很相似,反之,同源性有差异的,在生物特性上也表现出一定差异。3 isolates of P.infestans from tomato that couldn't infect ptato,3 isolates from potato that couldn't infect tomato and 2 isolates of P.infestans that could infect both tomato and potato was amplified using polymerase chair reaction(PCR) and the universal primers internal transcribed spacer ITS 1 and ITS4.PCR products wee an approximately 850 base bair and checked the sequences. The sequences of ribosomal DNA-ITS of 8 isolates were compared with 3 sequence logging in genebank and their homegenies were 100%. The homologous character of 3 isolates from tomato were 98.22% and 3 isolates from potato were 99.52%. The homogenies of 2 isolates that couldn't cross infect potato and tomato were 98.28%. The homogenies of 2 isolates that could infect tomato and potato were 99.17%,it was higher than isolates that couldn't cross infect. The resembling sequences leaded to the resemble biological characters. On the contrary, the different sequences leaded to different biological characters.

关 键 词：晚疫病菌 交叉侵染 ITS区段序列 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]