同种带瓣大动脉灭菌实验研究  

Experimental Study on Sterilizing of Conduit Valved Homograft

作　　者：余海彬[1] 冯德广[1] 赵根尚[1] 初佩俊[1] 蒋素娟[1] 郭龙辉[1] 李红卫[1] 徐成阳[1] 

机构地区：[1]郑州大学第二附属医院心血管外科

出　　处：《医药论坛杂志》2005年第4期40-42,共3页Journal of Medical Forum

摘　　要：目的探讨不同抗生素水浴灭菌方法对同种带瓣大动脉组织活性的影响。方法取带瓣大动脉30只,随机分为A、B、C实验组,每组10只,并从中随机选择10只剪取部分内膜组织作为对照组(D组),实验组用不同抗生素水浴灭菌方法处理并做细菌培养。各组测定葡萄糖代谢率和台盼蓝拒染率。结果实验组细菌培养无阳性且葡萄糖代谢率和台盼蓝拒染率均低于对照组(P<0.05),B、C两组葡萄糖代谢率和台盼蓝拒染率均高于A组(P<0.05),而B、C两组间葡萄糖代谢率和台盼蓝据染率差别无显著性(P>0.05)。结论同种带瓣大动脉在抗生素溶液中37℃水浴6h可达到满意的灭菌效果且对组织细胞活性损伤较轻。Objective To study the influence of different sterilization method in antibiotic solution to the viability of the conduit valved homograft.Methods 30 sheared conduit valved homofrafts were randomly divided into three experimental groups.group A、group B and group C,each of them contained 10 homografts.Randomly select the unsterilized tissue of 10 homografts as control group(group D).Experimental groups were undergone different sterilizstion method in solution and then germiculture was done.All groups were measured glucose utilization and non-Typan's dry rate.Results After sterilization,three experimental groups undergong germiculture have no postive case.The glucose utilization and non-Typan's dry rate of all experimental groups were lower evidently than that of the control group(P<0.05).Among the experimental groups,the glucose utilization and non-Typan's dry rate of group A were lower evidently than that of group B and group C(P<0.05),but there was no evident difference between group B and group C(P>0.05)。Conclusion Cultured in the 37 ℃ circumstance for 6h in antibiotic solution,conduit valved homograft can obtain the minimum damage to it's viability and the satisfying sterilizing effect.

关 键 词：同种带瓣大动脉 活性 灭菌 

分 类 号：R654.3[医药卫生—外科学]