抗体包被免疫磁珠分离CD4+T淋巴细胞亚群的方法学研究和初步应用  

Method evaluation and preliminary application of antibody-coating immunomagnetic beads to isolate CD4+ T lymphocyte subpopulation

作　　者：王岚[1] 金焕英[1] 王萃[1] 吴玲玲[1] 戴铁颖[1] 樊丹君[1] 郑筱叶[1] 

机构地区：[1]浙江大学医学院传染病研究实验室,浙江杭州310000

出　　处：《中国感染控制杂志》2005年第2期112-114,共3页Chinese Journal of Infection Control

摘　　要：目的　应用抗体包被免疫磁珠分离CD4 +T淋巴细胞亚群,并检测其效果。方法　运用直接包被法制备免疫磁珠,并以流式细胞仪分析。结果　CD4 +T淋巴细胞亚群分离率>95 %。结论　用直接包被法制备的免疫磁珠具有较好的分离CD4 +T淋巴细胞亚群效果。Objective To isolate CD4+ T lymphocyte subpopulation by antibody-coating immunomagnetic beads and evaluate the efficacy of detective methods. Methods Immunomagnetic beads were prepared by direct antibody coating and analysed by flow cytometry. Results The isolation rate of CD4+ T lymphocyte subpopulation was above 95%. Conclusion Direct antibody coating of immunomagnetic beads can isolate CD4+ T lymphocyte subpopulation effectively.

关 键 词：免疫磁珠 CD4+T淋巴细胞亚群 直接包被法 方法 

分 类 号：R392.11[医药卫生—免疫学]