人原始生殖细胞的迁移及表面标志物变化的实验研究  

Experimental study on migration of human primordial germ cells and changes of cell surface markers

作　　者：刘永刚[1] 陈地龙[1] 李芳菲[1] 王莎莉[1] 王亚平[1] 

机构地区：[1]重庆医科大学基础医学院组织胚胎学教研室,重庆400016

出　　处：《第三军医大学学报》2005年第8期716-719,共4页Journal of Third Military Medical University

基　　金：国家教育部重点科技项目 ( 0 110 9) ;重庆医科大学科研创新基金(CX2 0 0 10 2 ) ;重庆医科大学科研启动基金 (QP2 0 0 2 0 3)~~

摘　　要：目的　探讨人胚胎原始生殖细胞向生殖嵴迁移及其细胞标志物改变。方法　人胚生殖嵴或生殖腺经石蜡切片;HE染色,光镜观察;免疫组化方法检测阶段特异性胚胎抗原SSEA 4和SSEA 1的表达;钙 钴法检测碱性磷酸酶(AP)活性;RT PCR检测4 5、7、9、11周人胚胎生殖嵴中转录因子Oct 4的表达。结果　人胚胎第4.5周生殖嵴未见有碱性磷酸酶、阶段特异性胚胎抗原SSEA 4、SSEA 1阳性细胞,且不表达转录因子Oct 4;第5～11周胚胎生殖嵴中持续存在碱性磷酸酶、阶段特异性胚胎抗原SSEA 4、SSEA 1阳性细胞,且表达转录因子Oct 4;第12周胚胎生殖嵴中阶段特异性胚胎抗原SSEA 4、SSEA 1阳性细胞数量明显减少,但存在碱性磷酸酶阳性细胞。结论　人胚胎原始生殖细胞自第5周后开始迁移至生殖嵴,直至第11周后完全分化,其形态学特征为碱性磷酸酶、阶段特异性胚胎抗原SSEA 4、SSEA 1阳性,且表达转录因子Oct 4。Objective To investigate the migration of human primordial germ cells and changes of cell surface markers. Methods The gonadal ridges from 4.5 to 12-week human embryos were embedded with paraffine and sliced, stainded with HE and observed by light microscope. The slices of gonadal ridges were analyzed for the expression of alkaline phosphatase and immunologic markers (SSEA-1, SSEA-4) that were used routinely to characterize embryonic germ cells. The expression of transcriptional factor Oct-4 was detected by RT-PCR. Results There were no primordial germ cells in gonadal ridges in 4-week human embryos. The cell surface markers analyzed showed that the gonadal ridges possessed high levels of AP activity, were stained strongly for SSEA-4, SSEA-1 and expressed the transcriptional factor Oct-4 highly from 5 to 11-week embryos. Conclusion It is at the end of 5 week after fertilization that primordial germ cells migrate to gonadal ridges in human embryos. The primordial germ cells finish differentiation till 11 weeks after fertilization.

关 键 词：原始生殖细胞 生殖嵴 人 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学] R329.1[医药卫生—基础医学]