同种异体神经干细胞移植治疗大鼠脑内出血的研究  

The curative effect of the allograft of neural stem cells on experimental intracerebral hematoma in rats.

作　　者：张朋奇[1] 姜晓兵[1] 朱贤立[1] 赵甲山[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院神经外科,武汉430022

出　　处：《华中医学杂志》2005年第2期116-118,共3页Central China Medical Journal

摘　　要：目的　探讨神经干细胞移植对实验性大鼠脑内血肿的治疗作用。方法　脑内注血制作脑出血模型;乳鼠脑组织分离培养神经干细胞;细胞免疫组化鉴定细胞;血肿周围多点注射法移植神经干细胞;神经系统评分、正电子发射计算机断层扫描测脑组织代谢等方法衡量神经干细胞移植后治疗效果。结果　移植组与对照组相比神经系统评分无显著差异(P>0.05),移植组血肿周围脑组织代谢显著高于对照组相应脑区(P<0.01)。结论　移植的神经干细胞在大鼠脑内血肿周围能够成活,但不能改善神经功能缺失的症状。Objective To study the treatment effect of neural stem cells on the experimental intracerebral hematoma in rats.Methods A medium volume of hematoma was formed by stereotactic infusing 50 μl autologous caudal artery blood to right caudate nucleus. Neural stem cells from newborn rats' cerebra were established. The neural stem cells were identified by immunochemistry which expressing nestin antigen. The neural stem cells were transplanted at the area around the intracerebral hematoma in the adult rats by multi-spot injection. The behavioral were studied by Ya Hua's vibrissae-elicited forelimb placing test. Brain's metabolism was measured by PET(positron emission computerized tomography)scanner.Results The transplanted group is the same as the control group in Ya Hua’s vibrissae-elicited forelimb placing test(P> 0.05 ). In terms of the bran’s metabolism around hematoma, the transplanted group were markedly higher than that of the control group, showing significant difference (P< 0.01 ).Conclusion The neural stem cells can survival around the area of intracerebral hematoma, but there is no curative effect on experimental intracerebral hematoma in rats after the allograft of neural stem cells.

关 键 词：同种异体神经干细胞移植 治疗 大鼠 脑内出血 

分 类 号：R651[医药卫生—外科学]