绿僵菌属血清学的初步研究  

PRELIMINARY STUDY ON SEROLOGY OF METARHIZIUM

作　　者：胡锦江[1] 樊美珍[1] 高小满 

机构地区：[1]西北林学院,陕西杨陵712100

出　　处：《真菌学报》1994年第1期65-70,共6页

摘　　要：本文采用凝集试验和免疫电泳的方法对不同来源的七株绿僵菌属真菌进行了免疫学对比研究。抗原是孢子悬液和菌丝体清液,通过对家兔接种抗原而获得抗血清。每一种抗原与其同源抗血清和异源抗血清进行交叉试验,并对凝集试验的结果以及免疫电泳反应产生的沉淀弧数目和免疫电泳图谱加以对比分析,试验结果表明:不同菌株间存在着明显的抗原类似性,同时各菌株间也表现出一定的抗原专一性。根据试验数据对供试菌株进行血清学分型的结果与形态学分类相符合。Immunological studies of Metarhizium strains from different places and hosts were made by using immunoelectrophoresis and agglutination reaction. Antigens were conidium suspension and mycelium solution. Antisera were produced in rabbits from each of the 7 fungal strains. Each antigen was tested against the homo logous and heterologous antisera. The results of agglutination and the number of precipitin arcs obtained from immunoelectrophoresis were analyzed. These tests revealed numerous antigenic similarities between different strains of the same species. The grouping of strains obtained from immunoelectrophoretic data corresponds with that based on morphology.

关 键 词：绿僵菌 抗原 抗血清 昆虫真菌 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]