白色念珠菌诱导小鼠胸腺细胞凋亡通路的探究  

A study on pathway of apoptosis in murine thymocytesinduced by candida albicans

作　　者：暨明[1] 韦超凡[2] 陈欲晓[2] 蒋洪敏[3] 

机构地区：[1]中南大学湘雅医学院生理学系,长沙410078 [2]中南大学湘雅医学院免疫学教研室,长沙410078 [3]中南大学第二附属医院检验科,长沙410078

出　　处：《中国免疫学杂志》2005年第4期251-253,共3页Chinese Journal of Immunology

摘　　要：目的:探讨白色念珠菌诱导小鼠胸腺细胞凋亡的通路。方法:经小鼠尾静脉注射白色念珠菌后,用ELISA检测小鼠血清TNF -α水平;采用荧光分光光度计检测小鼠胸腺凋亡细胞caspase 3在不同时间组的活性变化;流式细胞仪检测小鼠胸腺细胞bax、p5 3、bcl 2基因产物水平。结果:TNF -α水平显著升高,caspase 3的活性明显增强,bax、p5 3基因表达上调。结论:白色念珠菌可能通过刺激机体TNF -α水平升高进而激活caspase 3,同时白色念珠菌上调bax、p5 3基因表达协同激活caspase-3,从而导致小鼠胸腺细胞凋亡。Objective:To investigate the pathway of C.albicans-induced murine thymocyte apoptosis.Methods:The chages of TNF-α level in murine plasma and caspase-3 activity were measured by ELISA and flurorescence spectrophoto meter respectively;FCM measurement was adopted for the expression levels of related genes.Results:The level of TNF rise,the caspase-3 activity serengthen significantly and bax,p53 gene expression are upregulated.Conclusion:It is possible that C.albicans elicits apoptosis in murine thymocytes by upregulating bax and p53 gene expression as well as raising the serum TNF-α level to strenghen the activity of caspase-3.

关 键 词：白色念珠菌 胸腺细胞 凋亡 TNF-α CASPASE-3 BAX p53 

分 类 号：R392[医药卫生—免疫学]