白藜芦醇对活化小鼠T淋巴细胞IL-2和CD25表达的影响  

The inhibition effects of resveratrol on IL-2 and CD25 expression in mouse lymphocytes

作　　者：俞瑜[1] 曾耀英[1] 刘良[2] 季煜华[1] 肇静娴[1] 

机构地区：[1]暨南大学组织移植与免疫教育部重点实验室,广州510632 [2]香港浸会大学中医药学院

出　　处：《中国免疫学杂志》2005年第4期279-282,共4页Chinese Journal of Immunology

基　　金：香港特区政府大学教育资助委员会基金 (2 14 4 0 3M ) ;广东省自然科学基金项目 (0 43 0 0 65 3 )资助

摘　　要：目的:研究白藜芦醇(RSV)对活化的小鼠T淋巴细胞分泌IL 2和CD2 5的影响,并探讨其免疫抑制机制。方法:无菌分离小鼠淋巴结细胞,与不同浓度的白藜芦醇共同孵育1小时后,用多克隆刺激剂佛波醇酯和离子霉素刺激T细胞活化,继续培养6小时后收获细胞,进行胞内细胞因子染色,流式细胞仪检测IL- 2的分泌情况,RT- PCR检测IL -2mRNA的表达;2 4小时后检测CD2 5表达。结果:RSV对IL 2的分泌具有抑制作用,并呈剂量依赖性,同时RSV也能抑制T细胞表面活化分子CD2 5。结论:RSV对活化T细胞分泌的细胞因子IL- 2及IL- 2α链CD2 5的表达可能是RSV对T细胞具有免疫抑制作用的机制之一,并可能与T细胞活化通路的PKC NF -κB信号传导途径相关。Objective:To study on the effects of resveratrol on IL-2 and CD25 expression in murine T activated lymphocytes.Methods:The lymphocytes were isolated and pre-treated with resveratrol for 1 h prior to activation with PDB plus ionomycin. After 6 hours stimulation,IL-2 secretion was determined by intracellular cytokine staining by flow cytometry. IL-2 mRNA expression was detected by RT-PCR. CD25 expression is determined by multi-color flow cytometric analysis after 24 hours stimulation.Results:RSV can inhibit IL-2 expression at mRNA and protein level. It also inhibit CD25 expression.Conclusion:The immunosuppressive mechanism of RSV maybe relative to the inhibitory effects on IL-2 mRNA and protein expression and CD25 expression, which may be relative to the T cell activated signal transduction of PKC-NF-κB pathway.

关 键 词：白藜芦醇 白细胞介素-2 CD25 

分 类 号：R392112[医药卫生—免疫学]