鸭跖草叶点霉的致病性与dsRNA及RAPD类群的相关分析  

Relationship between pathogenicity of Phyllosticta commelimecola of Commelina communis with dsRNA and RAPD groups

作　　者：胡汉桥[1] 于莉[1] 李赤[1] 张锐[2] 

机构地区：[1]湛江海洋大学农学院,广东湛江524088 [2]湛江海洋大学生物化学中心,广东湛江524088

出　　处：《华南农业大学学报》2005年第2期35-38,共4页Journal of South China Agricultural University

基　　金：湛江海洋大学学校课题资助

摘　　要：用随机引物扩增多态性(RAPD)和纤维素法对鸭跖草叶点霉Phyllostictacommelimecola的基因组遗传多样性和dsRNA进行测定,分析遗传多样性和dsRNA与菌株的致病力的关系.结果表明菌株间存在明显的培养类型和致病力分化现象,菌株的致病力从南到北逐渐减弱.选择东北地区有代表性的菌株,进行dsRNA测定和RAPD分析,在所测定的菌株中有10个含有dsRNA ,2个不含dsRNA ,菌株致病力强弱与dsRNA无相关性.通过RAPD扩增和聚类分析,在类间距离15以下可将12个鸭跖草叶点霉分为5个类群.辽宁省和吉林省的菌株亲缘关系较近,黑龙江省的菌株与辽宁、吉林的菌株亲缘关系远。The genetic polymorphism and dsRNA of Phyllosticta commelimecola from Northeastern of China were determined by RAPD and cellulose methods. The relationship between the genetic polymorphism and dsRNA and pathogenicity was analyzed. The results showed that there were obvious differences in culture types and pathogenicity of these strains, and the pathogenicity of the strains decreased from southern to northern. The typical strains were subject to dsRNA and RAPD analyses. Among 12 strains tested,10 strains contained dsRNA and 2 without dsRNA. It was mdicated that there was not relationship between dsRNA and pathogencity. The isolates could be divided into 5 groups under the Hierarchical cluster distance of 15. There was close relationship between the isolates of Jilin Province and Liaolin Province, however, the strains of Helongjiang Province had distant relationship with the strains of Jilin and Liaolin Province. The pathogenicity of the strains under the same RAPD group was similar.

关 键 词：鸭跖草 叶点霉 DSRNA RAPD 聚类分析 致病性 遗传多样性 杂草 生物防治 

分 类 号：S451.1[农业科学—植物保护] S476.1