重组pET30a/hsBLyS质粒在BL21(DE3)菌株中的稳定性观察  

Study on the heredity stability of a recombinant plasmid pET30a/hsBLyS in E.coli BL21(DE3)

作　　者：吴玉荣[1] 吴海涛[1] 曹萌[1] 曹鹏[1] 张双全[1] 

机构地区：[1]南京师范大学江苏省分子医学生物技术重点实验室,江苏南京210097

出　　处：《信阳师范学院学报（自然科学版）》2005年第2期165-168,共4页Journal of Xinyang Normal University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(30270193)

摘　　要：将构建的含有pET30a/hsBLyS重组质粒的BL21 (DE3)菌种,在含硫酸卡那霉素的平板培养基划线连续传代至 100代 100代次菌种经显微观察,为典型的革兰氏阴性大肠杆菌,生化特性没有改变 提取0、50、100代质粒DNA限制性内切酶切割检查,酶切图谱没有改变,DNA测序未见hsBLyS基因变异 传代前后菌种诱导表达hsBLyS。The strain of pET30a/hsBLyS/BL21 (DE3)had been subcultured consecutively from the original stock to the 100^(th) generation on LB plates with kanamycin.The digestion products of plasmid in the original ,the 50^(th) and the 100^(th) generation by NdeⅠand BamHⅠrestriction enzymes are same.As well,DNA sequences of the hsBLyS gene shows no differences among the generations.In addition to that,the original strain and the 100th generation strain have a similar hsBLyS expression level and these hsBLyS proteins show similar activites.

关 键 词：BLYS 工程茵种 传代 遗传稳定性 

分 类 号：Q78[生物学—分子生物学]