芦根液相色谱指纹图谱研究  

Fingerprint of Rhizoma Phragmitis by HPLC

作　　者：曾志[1] 张艳萍[1] 李核[1] 杨挺[1] 朱兰[1] 赵富春[1] 

机构地区：[1]华南师范大学化学系分析测试中心,广东广州510631

出　　处：《中成药》2005年第4期373-377,共5页Chinese Traditional Patent Medicine

基　　金：广东省科技计划项目重大专项(No.A301020101);广东省自然科学基金项目 (No. 011442 );广东省教育厅高校自然科学研究项目(No. 0124);广东省中医药局科研项目(No. 403047; 401039)资助

摘　　要：目的:研究芦根药材的指纹图谱,为含芦根中药制剂制定基本的指纹图谱。方法:利用液相色谱法分析芦根丙酮提取物,采用反相ODS 3柱,流动相: 1 醋酸溶液 5 乙腈溶液 =3∶7(V∶V),检测波长为 250nm。结果:根据相对保留值α和相对峰面积Sr建立了芦根药材的指纹图谱。结论:实验结果表明该法灵敏度高,重现性好。AIM: To provide the applied ground of the formulation of Rhizoma Phragmitis by setting up a fingerprint of Rhizoma Phragmitis. METHODS:We gained the extracts of Rhizoma phrapmitis by acetone extraction and assayed its extracts by means of HPLC, with a mobile phase of water (1% acetic acid)-water(5% acetonitrile) on inertsil ODS-3 column. an ultraviolet detector was at 250 nm. RESULTS:Fingerprint was established on the basis of the relative retention value and the relative peak area. CONCLUSION: The method is of high sensitivity and good reproducibility.

关 键 词：芦根 高效液相色谱 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]