正交实验法优化大黄蒽醌超临界流体萃取的研究  

Investigation of orthogonal design of supercritical fluid extraction(SFE) for extracting anthraquinone aglycones from Rhubarb

作　　者：刘玉敏[1] 陈闽军[2] 洪筱坤[1] 

机构地区：[1]上海中医药大学,上海201203 [2]上海交通大学药学院,上海200030

出　　处：《中成药》2005年第4期386-389,共4页Chinese Traditional Patent Medicine

摘　　要：目的:优化SFE法提取大黄蒽醌工艺。方法:用正交设计法和方差分析考察影响超临界流体萃取 5个因素及最佳萃取条件。结果:实验所得SFE最佳萃取条件为:温度 70°C、压力 35Mpa、甲醇剂量0. 6mL、静态萃取时间 8min及动态萃取体积 5mL。结论:SFE技术具有萃取效率高、速度快、操作简便等优点,可用于大黄蒽醌的提取分离。AIM: To optimize the extraction of the anthraquinone aglycnes from Rhubarb using supercritical fluid extraction(SFE). METHODS: Orthogonal design and variance analysis were used to optimize five operational variables of SFE. RESULTS: The optimized operational variables of SFE were obtained as following: the temperature was 70 °C , the pressure was 35 MPa, the static extraction time was 8 min, the dynamic extraction volume 5 mL and methanol were used as modifier. CONCLUSION: SFE is rapid, convenient and accurate, and can be used to extract athraquinone aglycones from Rhubarb.

关 键 词：正交设计法 大黄蒽醌化合物 超临界流体萃取 

分 类 号：R284.2[医药卫生—中药学]