RP-HPLC测定复方当归妇康胶囊中丹参酮ⅡA的含量(英)(英文)  

Determination of tanshinone ⅡA in Compound Dangguifukang Capsule by RP-HPLC

作　　者：王新春[1] 于志庚[1] 陈卫军[1] 胡志林[1] 

机构地区：[1]新疆石河子大学医学院第一附属医院,新疆石河子832008

出　　处：《中成药》2005年第4期424-426,共3页Chinese Traditional Patent Medicine

基　　金：ShiheziUniversityofNaturalScienceFundCommittee, itemnumber: 2001 822574

摘　　要：目的:建立HPLC法测定复方当归妇康胶囊中丹参酮ⅡA含量的方法.方法:采用ODS柱,以甲醇-水(80:10)为流动相,检测波长为270nm,流速为1.0mL·min^-1.结果:丹参酮ⅡA在0.01～0.16μg,范围内呈良好线性关系,丹参酮ⅡA平均回收率99.73%,RSD为2.91%(n=5).结论:该方法简便,分离完全,结果可靠,适用于该制剂的质量控制.AIM: To establish a HPLC method for the determination of tanshinone ⅡA in Compound Danguifukang Capsule. METHODS: Analytic column: Reliasil C 18 (5 μm, 250 mm×4.6 mm), protective column:(5 μm, 12.50 mm×4.6 mm); mobile phase: methnaol-ultrapure water (80∶20); detective wavelength was at 270 nm; temperature was at 30 °C ; the flow rate was about 1.0 mL·min -1 . RESULTS: The standard curve showed a good linearity within the range of 1×10 -2 -0.16 μg, r=0.999 5. The average recovery was 99.73% and RSD was 2.91% . CONCLUSION: The method is feasible, and simple to operate and suitable for the quality control of Compound Dangguifukang Capsule.

关 键 词：HPLC 当归妇康胶囊 丹参酮ⅡA 

分 类 号：R927[医药卫生—药学]