肠安康胶囊对实验性结肠炎的作用机理探讨  

Effect of Chang'ankang Capsule on rat's colitis and its mechanism

作　　者：王献坤[1] 邢建峰[1] 郑晓鹤[1] 侯家玉[1] 

机构地区：[1]北京中医药大学中药学院中药药理系,北京100102

出　　处：《中成药》2005年第4期443-446,共4页Chinese Traditional Patent Medicine

基　　金：国家中医药管理局新药开发专项(DIX034A)

摘　　要：目的:观察肠安康胶囊(黄芪、黄连、金银花等 )对大鼠溃疡性结肠炎的治疗作用,并探讨其机理。方法:三硝基苯磺酸乙醇溶液大鼠灌肠建立溃疡性结肠炎模型,一周后给予治疗药物,连续 14d。造模第 21天,观察大鼠结肠大体和病理组织学损伤情况,并检测髓过氧化物酶(MPO)、一氧化氮 (NO)、超氧化物歧化酶 (SOD)、丙二醛 (MDA)、谷胱苷肽过氧化物酶(GSH PX)、过氧化氢酶(CAT)活性或含量的变化。结果:模型组大鼠结肠大体评分、病理组织学和生化指标表明模型复制成功,肠安康胶囊明显改善上述指标,并显著降低MPO、NO、MDA的活性或含量,升高SOD、GSH PX、CAT的活性。结论:肠安康胶囊通过抗氧自由基损伤,抑制NO的生成,减少炎细胞浸润,对溃疡性结肠炎有明显的保护作用。AIM: To observe the effect of Chang'ankang Capsule (Radix Astragali, Rhizoma loptidis, Flos Lonicerae, etc.) on rat's colitis induced by trinitrobenzene sulfonic acid (TNBS) and investigate its mechanism. METHODS: The rat's colitis was induced by TNBS and ethanol, one week later the rats were given the medicine for 14 days. The 21st day the mucosal damage index (CMDI) and histopathology (HP) of the colon were observed. Meanwhile the level of myeloperoxidase (MPO), nitric oxide (NO), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), maiondiadehyde (MDA), catalase (CAT) were detected. RESULTS: CMDI and HP, biochemistry in model rats showed that the models were successful. Chang'ankang groups significantly alleviated CMDI, HP, and improved the high level of MPO, NO and MDA, promoted the content of SOD, GSH-PX, CAT. CONCLUSION: Treatment with Chang'ankang shows beneficial effects on rat's ulcerative colitis by alleviating damage of free radical and NO and leukocyte.

关 键 词：肠安康胶囊 溃疡性结肠炎 髓过氧化物酶 一氧化氮 超氧化物歧化酶 

分 类 号：R285.5[医药卫生—中药学]