半边旗提取物5F对人高转移卵巢癌细胞HO-8910PMETS-1mRNA和VEGF蛋白表达的影响  

Effects of 5F from Pteri semipinnata on the Expression of ETS-1mRNA and VEGF Protein of Highly Metastatic Ovarian Carcinoma HO-8910PM Cells

作　　者：何太平[1] 莫丽儿[2] 梁念慈[1] 

机构地区：[1]广东医学院生物化学与分子生物学研究所,广东湛江524023 [2]广东天然药物研究与开发重点实验室,广东湛江524023

出　　处：《中药材》2005年第4期307-309,共3页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金资助课题;No39870900;湛江市988科技兴湛计划项目

摘　　要：目的:研究半边旗提取物5F(以下简称5F)对HO-8910PM细胞内VEGF蛋白及ETS-1mRNA表达的影响,探讨其抗肿瘤侵袭转移的作用机制.方法:RT-PCR分析ETS-1mRNA的表达;Western blot分析VEGF蛋白的表达.结果:25～100 μmol/L 5F作用HO-8910PM细胞24 h后,明显下调ETS-1mRNA的表达;5F明显下调VEGF蛋白表达水平.结论:5F抗肿瘤侵袭转移能力与ETS-1mRNA和VEGF蛋白的表达有关.Objective:To study the effect of 5F from Pteri semipinnata L on the expression of ETS-1mRNA and VEGF protein,and to investigate the antitumor mechanisms of 5F from Pteri semipinnata L.Methods:RT-PCR assay was used to assess the expression level of ETS-1 mRNA.The expression level of VEGF protein was assessed by Western blot analysis.Results:The expression level of of VEGF protein obviously decreased in HO-8910PM cells treated with 25～100 μmol/L 5F for 24 hours.5F significantly down-regulated the expression of ETS-1mRNA.Conclusion:The antitumor activity of 5F from Pteri semipinnata L is involved in the expression of ETS-1 mRNA and VEGF protein.

关 键 词：半边旗提取物 卵巢癌细胞 5F 高转移 HO-8910PM VEGF蛋白 mRNA表达 肿瘤侵袭转移 WESTEM 蛋白表达水平 ETS-1 PCR分析 mol/L 抗肿瘤侵袭 作用机制 blot 转移能力 细胞内 RT- 24h M细胞 

分 类 号：R965[医药卫生—药理学] R737.31[医药卫生—药学]