丙烯腈对大鼠睾丸抗氧化酶活力和脂质过氧化水平的影响  

Effects of acrylonitrile on the activities of antioxidant enzymes and levels of lipid peroxidation in rat testes

作　　者：黄简抒[1] 钟先玖[1] 吴鑫[2] 金泰廙[2] 

机构地区：[1]复旦大学附属金山医院职业病科,上海200540 [2]复旦大学附属金山医院公共卫生系,上海200540

出　　处：《中华劳动卫生职业病杂志》2005年第2期136-138,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的　探讨丙烯腈(ACN)的雄性生殖毒性机制。方法　健康雄性SD大鼠分别以0、7.5、15.0、30.0 mg/kg剂量腹腔注射ACN染毒,测定其睾丸谷胱甘肽(GSH)、丙二醛(MDA)含量和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH Px)、谷胱甘肽S 转移酶(GST)活力。结果　ACN染毒4周后,15.0、30.0 mg/kg组大鼠睾丸GSH含量[(7.44±0.96)、(6.95±0.77)mg/g pro]增加,30.0 mg/k组大鼠GSH Px活力[(70.89±4.01)U/mg pro]升高,与对照组的差异均有统计学意义(P<0.05,P<0.01)。ACN染毒8周后,30.0 mg/kg组大鼠睾丸GSH含量[(2.50±0.94)mg/g pro]降低,15.0、30.0mg/kg组大鼠SOD活力[(102.08±16.08)、(113.30±17.20)NU/mg pro]升高,与对照组的差异均有统计学意义(P<0.01)。ACN染毒13周后,15.0、30.0 mg/kg组大鼠睾丸GSH含量降低,30.0 mg/kg组GST活力下降,各染毒组GSH Px活力均低于对照组,30.0 mg/kg组MDA含量[(0.68±0.16)nmol/mgpro]高于对照组[(0.38±0.12)nmol/mg pro],差异均有统计学意义(P<0.01)。停止染毒后2周,大鼠睾丸GSH水平恢复;30.0 mg/kg组大鼠睾丸MDA含量下降,但仍高于对照组,GSH Px活力仍低于对照组,差异均有统计学意义(P<0.05)。结论　ACN能通过破坏氧化-抗氧化体系的平衡,诱导雄性大鼠睾丸脂质过氧化损伤。Objective To explore the mechanism of male reproductive toxicity induced by acrylonitrile(ACN). Methods Male Sprague-Dawley rats were daily administrated ACN by intraperitoneal injection 5 times a week for 13 weeks at the dose of 0,7.5,15.0 and 30.0 mg/kg body weight,respectively.The rats were sacrificed and testes were removed at the end of 4,8,13 or 15 weeks,respectively.The activities of glutathione peroxidase(GSH-Px),superoxide dismutase(SOD) and glutathione S-transferase(GST) and the levels of glutathione(GSH) and malonaldehyde(MDA) were detected in testes. Results Following ACN treatment of 4 weeks,the levels of GSH in ACN 15.0 mg/kg and 30.0 mg/kg group were (7.44±0.77)mg/g pro and (6.95±0.77)mg/g pro respectively,and the activity of GSH-Px was (70.89±4.01)U/mg pro in 30.0 mg/kg group,all of which were significantly higher than the control group(P<0.05,P<0.01).After 8 weeks,the levels of GSH decreased to (2.50 ±0.94)mg/g pro in ACN 30.0 mg/kg group(P<0.01);the activities of SOD increased to (102.08± 16.08) NU/mg pro and (113.30±17.20)NU/mg pro in ACN 15.0 mg/kg and 30.0 mg/kg group(P<0.01).After 13 weeks,the levels of GSH declined in ACN 15.0 mg/kg and 30.0 mg/kg group,and the activities of GST decreased in ACN 30.0 mg/kg group,and of GSH-Px decreased in both doses group.However,the level of MDA[(0.68±0.16)nmol/mg pro] were significantly higher in 30.0 mg/kg group than that in control group[(0.38± 0.12) nmol/mg pro](P<0.01).2 weeks after stopping ACN treatment,the level of GSH restored to normal but the levels of MDA or the activity of GSH-Px in 30.0 mg/kg group were still higher or lower respectively than those of control(P<0.05). Conclusion ACN may impair the balance of antioxidant system,thus induce lipid peroxidation damage to rat testes.

关 键 词：丙烯腈 大鼠 睾丸 抗氧化酶活力 脂质过氧化 

分 类 号：R114[医药卫生—卫生毒理学]