艾滋病病毒1型流行株分子流行病学调查  

Molecular epidemiological investigation on HIV-1 epidemic strain

作　　者：颜瑾[1] 王玉[2] 李杰[1] 马鹏飞[2] 林鹏[1] 邢辉[2] 刁丽梅[1] 邵一鸣 

机构地区：[1]广东省疾病预防控制中心微生物检验所,广州510300 [2]中国疾病预防控制中心性病艾滋病预防控制中心

出　　处：《中国公共卫生》2005年第1期90-91,共2页Chinese Journal of Public Health

基　　金：广东省医学科学技术研究基金(WSTJJ20011226440105197202120061)

摘　　要：目的了解广东省HIV1亚型的流行规律及其与国际参考株的同源性。方法应用套式聚合酶链反应(PCR)对108例广东省HIV1感染者淋巴细胞富集液的核酸样品进行扩增,并使用ABI377型测序仪对扩增产物测序后,对其外膜蛋白(ENV)基因C2V3段的核酸序列进行比较分析。结果108份血样中,44份为AE重组亚型,与国际参考株01AE.TH.90.CM240最近,基因离散率为(8.644±1.273)%,组内离散率为(7.302±4.639)%;40份为07BC重组亚型,与国际参考株CN.97.C54A最近,基因离散率为(2.163±1.313)%,组内离散率为(3.388±1.686)%;14份为08BC重组亚型,与国际参考株97CNGX9F最近,基因离散率为(2.757±2.027)%,组内离散率为(3.728±1.971)%;10份为B亚型,其中9份为泰国B′,与国际参考株RL42最近,基因离散率为(5.687±2.232)%,组内离散率为(5.879±2.542)%;1份为欧美B,与国际参考株US.86.JRFL最近,基因距离为8.50%。结论广东省HIV1流行株以AE和07BC重组亚型为主,也存在08BC重组亚型、泰国B亚型和欧美B亚型。Objective To investigate the subtype of HIV-1 prevelent in Guangdong province.Methods HIV-1 pro-viral DNA from buffy coat of 108 infectors in Guangdong was amplified by nested PCR.The C2-V3 regions of HIV-1 ENV gene were sequenced directly from the PCR product and were analyzed.Results The 108 infectors were confirmed to be infected with five HIV-1 subtype or Circulating Recombinant Form(CRFs):AE(n=44),07-BC(n=40),08-BC(n=14),B′(n=9)and B(n=1).The AE group showed the highest homology to 01AE.TH.90.CM240 strain isolated in Thailand,and the genetic distances was(8.644±1.273)%.The 07-BC group showed the highest homology to CN.97.C54A strain,and the genetic distances was(2.163±1.313)%.The 08-BC group showed the highest homology to 97CNGX-9F strain,the genetic distances was(2.757±2.027)%.The B′ group showed the highest homology to r142 strain isolated in Thailand,and the genetic distances was(5.687±2.232)%.THe B subtype showed highest homology to US.86.JRFL strain isolated in USA,and the genetic distance was 8.50%.The innergroups distance among AE,07-BC,08-BC,B′ group were(7.302±4.639)%,(3.388±1.686)%,(3.728±1.971)%,(3.728±1.971)%,respectively.Conclusion AE and 07-BC were main epidemic subtypes in Guangdong province.08-BC,B′ and B were also circulating in Guangdong province.

关 键 词：吸毒者 艾滋病病毒(HIV)1亚型 序列测定 

分 类 号：R512.91[医药卫生—内科学]