纳洛酮对血管性痴呆大鼠学习和记忆能力的影响  

Effects of Naloxone on Learning and Memory Ability in Vascular Dementia Rats

作　　者：郑先振 杨艳珍 马光瑜[2] 

机构地区：[1]广东省精神卫生研究所,广州510120 [2]广东省食品药品监督管理局,广州515041

出　　处：《医药导报》2005年第5期361-363,共3页Herald of Medicine

基　　金：广东省自然科学基金资助项目(基金编号:000825)

摘　　要：目的研究纳洛酮对血管性痴呆大鼠空间学习和记忆能力减退的防治作用及作用机制。方法将30只SD大鼠随机分为模型组、给药组和对照组各10只。结扎模型组和给药组大鼠双侧颈总动脉,以制作血管性痴呆模型。模型制作成功后,给药组腹腔注射纳洛酮,0.8mg·kg1·d1;模型组腹腔注射等量0.9%氯化钠注射液。对照组大鼠给予假手术,不结扎双侧颈总动脉,腹腔注射等量0.9%氯化钠注射液。均连续给药7d。8周后进行Morris水迷宫试验,观察3组大鼠隐匿平台逃避潜伏期、去平台后每分钟穿越平台所在位置的次数、恢复平台后从一固定点爬上平台所用的时间,并做病理学检查。结果模型组大鼠隐匿平台逃避潜伏期[(21.26±7.41)s]明显长于对照组[(7.80±4.70)s]和给药组[(8.10±2.93)s](均P<0.01),但对照组和给药组之间差异无显著性;去除平台后,模型组大鼠每分钟穿越平台所在位置的次数[(4.44±1.74)次]明显少于对照组[(8.45±1.19)次]和给药组[(8.00±1.17)次](均P<0.01),对照组和给药组差异无显著性;恢复平台后3组大鼠从一固定点爬上平台所用的时间无明显差别;病理检查结果显示,模型组大鼠脑海马(CA)1区锥体细胞数[(43.68±17.29)个]较对照组[(124.51±14.18)个]和给药组[(113.36±13.15)个]明显减少(均P<0.01)。Objective To study the preventive effects and mechanism of naloxone on spatial learning and memory deficits in vascular dementia rats. Methods Thirty SD rats were randomised into model group, treatment group and control group. Each group had ten rats. The model group and the treatment group were made by bilateral occlusion of both common carotid arteries (2-VO method) to make the vascular dementia models. Then the treatment group received intraperitoneally naloxone treatment (naloxone hydrochloride, 0.8 mg·kg 1 ·d 1 ) immediately for 7 d after the operation; the model group received eaqual volume sodium chloride injection; and the control group were given same operation without ligation to both common carotid arteries, and received equal volume sodium chloride injection. After the operation for 8 wk, all of the rats were trained in Morris Water Maze (MWM) program for 5 d to find the escape latencies, times of going through the platform after removed the platform and the climbing time from a specific point to the platform when the platform were set back. Then pathology examination were performed. Results The escape latencies in model group was significant longer than that in the control group and treatment group ( P <0.01) , but there were no significant differences between the latter two groups. The times of passing through after the platform were removed in the model group were significant decreased than that in the control group and in the treatment group ( P <0.01), but the latter two groups had no significant differences.The escape latencies had no significant differences among three groups in the visible platform task training ( P >0.05). The pathological examination indicated that the number of pyramidal neurons in model group (43.68±17.29) were significant decreased than in the control group (124.51±14.18) and in the treatment group (113.36±13.15). Conclusion The preventive effects of naloxone on spatial learning and memory deficits in vascular dementia rats are significant. Its mechanism may be owing

关 键 词：纳洛酮 痴呆 血管性 空间学习能力 记忆能力 锥体细胞 

分 类 号：R971.5[医药卫生—药品] R965[医药卫生—药学]