铅对海马神经元单环磷酸腺苷水平的影响  

Effect of lead on cyclic AMP level in cultured hippocampal neuron

作　　者：徐健[1] 颜崇淮[2] 余晓刚 杜学良[1] 吴真[1] 沈晓明[1] 

机构地区：[1]上海第二医科大学附属新华医院,200092 [2]上海市儿科医学研究所

出　　处：《中国公共卫生》2005年第3期277-278,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金资助项目 (30 0 70 665) ;上海市教委科技启明星项目资助 (99QB1 4 0 0 8)

摘　　要：目的　通过放射免疫的方法 ,研究铅暴露对体外培养的海马神经元单环磷酸腺苷 (cyclicadenosinemonophosphatec,cAMP)水平的影响。 方法　在体外培养 3d的原代胚鼠海马神经元中 ,加入不同浓度的氯化铅 (分别为 10 -4mol/L、10 -6mol/L、10 -8mol/LPb2 + )暴露 2 1d ,收集海马细胞并超声粉碎后 ,用放射免疫的方法测海马细胞cAMP水平。结果　铅抑制体外培养的海马神经元腺苷酸环化酶活性 ,降低cAMP水平。 1× 10 -8mol/LPb2 + 暴露即能抑制海马细胞腺苷酸环化酶活性。铅暴露程度越高 ,cAMP降低越明显 ,两者成负相关。结论　铅暴露后海马神经元cAMP水平下降可能是铅影响学习记忆的机制之一。Objective To determine the effect of lead exposure on cyclic AMP level in cultured hippocampal neurons.Methods To establish the neuron model of lead exposure in vitro. After primary embryonal rat hippocampal neurons were cultured for 3 days,cells were induced by different concentration of Pb 2+ (10 -4 ,10 -6 ,10 -8 ?mol/L)for three weeks.To shatter hippocampal neurons with ultrasonic disintegrator and to determine the cyclic AMP level by radio-immunity assay.Results Lead could inhibit adenylate cyclase,even in lead exposure concentration of 1×10 -8 mol/L.Higher level of lead exposure led to lower cyclic AMP level,which showed a dose-dependent effect.Conclusion The descent of cyclic AMP level in hippocampal neurons exposed to lead maybe one mechanism for lead affecting learning and memory.

关 键 词：铅 海马 神经元 单环磷酸腺苷 

分 类 号：R135.1[医药卫生—劳动卫生] 1[医药卫生—公共卫生与预防医学]