用生物发光法对绿脓杆菌粘附因子的研究  

STUDIES ON THE ADHERENT FACTORS OF PSEUDOMONAS AERUGINOSA WITH BIOLUMINESCENCE

作　　者：孙嘉艳 程松高[1] 

机构地区：[1]首都医学院微生物学教研室

出　　处：《微生物学报》1989年第5期378-382,共5页Acta Microbiologica Sinica

基　　金：中国科学院基金

摘　　要：用生物发光法(BL)研究了绿脓杆菌(PA)表面介导及与聚苯乙烯管粘附的成分。结果说明,粘多糖(slime polysaccharied,SPS)是某些粘质(slime)型菌株的一个起粘附作用的重要因子。用来自PA3的SPS预先包被聚苯乙烯管可以增加经洗涤PA3、PA4和PA8的粘附量;PA3SPS抗血清可以抑制PA与聚苯乙烯管的粘附;N-乙酰葡萄糖胺(GlcNAc)具有抑制PA3SPS增加粘附的作用。A bioluminescence method was established for quantifying the adhesion of P. aeruginosa to polystyrene and the adherent components were investigated. The results indicated that the slime polysaccharide (SPS) is an important adherent factor of some slime strains of P. aeruginosa. The adhered amount of washed slime strains could be increased by pre-coating of polystyrene with SPS obtained from PA3. The activity of PA3SPS could be inhibited by anti-PA3SPS antiserum and blocked by N-acetylglucosamine.

关 键 词：生物发光法 绿脓杆菌 粘附因子 

分 类 号：R378.991[医药卫生—病原生物学]