浮游植物中叶绿素a提取方法的比较与改进  

Comparison and improvement on the extraction method for chlorophyll a in phytoplankton

作　　者：林少君[1] 贺立静[1] 黄沛生[1] 韩博平[1] 

机构地区：[1]暨南大学水生生物研究所,广州510632

出　　处：《生态科学》2005年第1期9-11,共3页Ecological Science

基　　金：教育部优秀青年教师基金

摘　　要：对水质监测中叶绿素a的提取方法进行改进,采用反复冻融-浸提方法提取叶绿素a,即将过滤水样的滤膜于-20℃冰箱内和室温下反复冻融3～5次后,放进90%丙酮溶液中浸提20h。与标准方法中的研磨法对比,该方法具有人为误差小、稳定性好、结果准确及操作简单安全等优点,适合运用于常规的水质监测。采用该方法时过滤水量对湖水水样的测定结果有影响(P<0.01),对于纯微囊藻液影响不明显(P>0.05)。因此,在对不同叶绿素水平或营养水平的水体采样时,应注意水样的水量。通过方差分析表明该方法中采用醋酸纤维滤膜、微孔滤膜和玻璃纤维滤膜对结果没有显著影响,对这3种滤膜可以进行自由选择。The extraction of chlorophyll a from phytoplankton was by freezing-thawing was assessed in this study. Filters containing alga cells were frozen and thawed repeatedly, then were steeped in 90% acetone solution for ~ 20 hours. Compared to the standard rubbing method, the freezing-thawing method had the improvements on small artificial error, high consistency, high accuracy, and operational simplicity. The filteration volume had no significant effect on the results for the mono-species algal cultures (P>0.05), but Bur the results for the lake water were significantly affected by filteration volume (P<0.01). For the water samples from eutrophic waterbodies, the 100ml water volume was suggested to be filtered.The data analysis showed there was no significant difference between Acetate Fiber Filter, Tiny Aperture Filter and Glass Fiber Filter.

关 键 词：叶绿素a提取 反复冻融 研磨 

分 类 号：X832[环境科学与工程—环境工程]