麦胚凝集素的分离纯化和鉴定  

Isolation and purification of wheat germ agglutinin and analysis of its properties

作　　者：王凯南[1] 朱琳云[1] 曲爱琴[1] 李守玲[1] 

机构地区：[1]山东大学生命科学学院生物技术系,山东济南250100

出　　处：《中国生化药物杂志》2005年第2期67-69,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的研究用鸡卵黏蛋白为配基亲和色谱法分离纯化麦胚凝集素,并对纯化的麦胚凝集素进行性质分析。方法先制备鸡卵黏蛋白的亲和吸附剂,然后用其纯化麦胚凝集素。结果此亲和色谱方法使纯化后的麦胚凝集素比其粗提液纯度提高约10 0 0倍,相对分子质量为2 0 4 2 0 ,pI为5 .10。PurposeTo make some research on the method of purification of wheat germ agglutinin (WGA) using Sepharose-4B which is combined with gallinaceous ovomucoid as an affinity adsorbent, and to analyze its properties.MethodsChromatographic column coupled with gallinaceous ovomucoid was used to isolate and purify WGA.ResultsThe hemagg1utinating activity of purified WGA was raised by about 1 000 times. The molecular weight of WGA was 20 420, and its isoelectric point is 5.10.ConclusionThis means of affinity chromatography with gallinaceous ovomucoid has been proven to be a kind of economical and applicable method to purify and isolate WGA.

关 键 词：麦胚凝集素 亲和色谱 分离 纯化 

分 类 号：Q513.2[生物学—生物化学]