RP-HPLC法测定广东蛇药有效成分柚皮苷的含量  

Determination of Narrigin in Citrus Grandis(L.) Osbeck by RP-HPLC

作　　者：杨燕军[1] 林洁红[1] 

机构地区：[1]广东省药物研究所,广东广州510440

出　　处：《中国中医药信息杂志》2005年第4期49-50,共2页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的建立广东蛇药主药柚叶中有效成分柚皮苷含量的测定方法。方法采用RP-HPLC法,固定相为HypersilBDSC18(5μm,4.6mm×250mm);流动相为甲醇-水-醋酸(35∶61∶4);流速为1.0mL/min;检测波长为283nm;柱温为室温;进样量为10μL。结果柚皮苷浓度在20～80μg/mL范围线性关系良好(r=0.999),平均回收率为98.1%,RSD=1.0%(n=6)。结论本方法简便、准确、重现性好。Objective To develop the analysis method to determine the content of narrigin in Citrus grandis(L.) Osbeck by reverse HPLC. Methods The chromatographic contition was Hypersil BDS C18 column (4.6 mm×250 mm, 5 μm). A mixture of methanol-water-acetic acid (35∶61∶4) was used as the mobile phase with a flow rate of 1.0 mL/min and the detection wavelength was 283 nm at room temperature. Results The calibration curve for narrigin showed good linear correlation within the concentration range of 20 ～ 80 μ g/mL (r =0.999). The average recovery was 98.1%, the precision RSD was 1.0%(n =6). Conclusion The method is convenient, reliable and with good reappearance.

关 键 词：广东蛇药 柚叶 柚皮苷 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]