微流控分析芯片在线尿中蛋白及LDH同工酶检测  

On-line detection of urinary protein and lactate dehydrogenase isoenzyme by capillary electrophoresis microchip

作　　者：金庆辉[1] 庄贵生[1] 刘菁[2] 贾春平[1] 王惠民[3] 赵建龙[1] 

机构地区：[1]中国科学院上海微系统与信息技术研究所,上海200050 [2]中国科学院研究生院,北京100039 [3]南通大学附属医院,江苏南通226001

出　　处：《光学精密工程》2005年第2期151-157,共7页Optics and Precision Engineering

基　　金：国家863计划项目(No.2002AA2Z2021;2004AA4042522)

摘　　要：利用MEMS(微机电系统)标准光刻、湿法腐蚀以及低温键合技术制作了微流控分析芯片。在自制的紫外吸收微芯片生化分析仪上,利用该芯片对临床妊娠高血压和多发性骨髓瘤患者的尿液样本进行了在线芯片电泳分析,得到了与医院常规诊断方法一致的诊断结果,而此法进行一次分析仅需要2 min,且试剂消耗量少,这是常规方法不能比拟的。也以LDH同工酶标准品为分析对象,对芯片在线同工酶诊断进行了初步研究,初步实现了LDH1 和LDH5 混合样本的片上分离、孵育反应和产物检测。The microfluidic chips were fabricated with standard photolithography, wet etching and low-temperature bonding techniques of MEMS (Micro-electro-mechanical system). Clinical urine samples from gestational hypertension syndrome and multiple myeloma patients were analyzed with the microfluidic chips on home-made UV absorption-based biochem-analysis instrument. The results are in accordance with those from standard urinary proteins assay method. The advantages of microchip-based urinary protein assays such as reduction in analysis time (2 min) and reagent consumption display great superiority over the standard urine assay method. Standard models of lactate dehydrogenase isoenzymes were also selected as the samples for initial study of on-chip LDH assay. The integration of on-chip enzyme separation, enzyme reaction and product detection for mixture of LDH1 and LDH5 was realized.

关 键 词：微流控分析芯片 毛细管电泳微芯片 尿蛋白 LDH同工酶 

分 类 号：Q657[生物学—生物物理学]