油菜素甾酮的^（125）I标记及其生物活性  

Preparation of ￣(125)I-Brassinosterone and Its Biological Activity

作　　者：徐如涓[1] 王玉琴[1] 吴登如 何宇炯[1] 赵毓橘[1] 方军[1] 

机构地区：[1]中国科学院上海植物生理研究所,上海中医学院

出　　处：《植物生理学报（0257-4829)》1994年第2期121-127,共7页Acta Phytophysiologica Sinica

摘　　要：油菜素甾酮（ｂｒａｓｓｉｎｏｓｔｅｒｏｎｅ）是合成油菜素内酯（ｂｒａｓｓｉｎｏｌｉｄｅ）的前体，它具有７０％以上的油菜素内酯生物活性。油菜素甾酮的Ｂ环－６－酮基经与盐酸羧甲基羟胺反应后得到油菜素甾肟，当浓度为１０μｍｏｌ／Ｌ时用水稻叶片倾斜法测得其生物活性比对照增加６０％以上；当浓度为２μｍｏｌ／Ｌ时能促使黄瓜下胚轴伸长，比对照增加２０％。用油菜素甾肟与油菜素内酯或油菜素甾酮一起处理黄化水稻幼苗，测得水稻叶片与叶路之间的倾斜角度介于它们单独处理之间。用氯胺－Ｔ法制备１２５Ｉ－组胺，再与油菜素甾肟结合。先用黄瓜幼苗下胚轴伸长法初步筛选活性组份，再用水稻叶片倾斜法鉴定活性，表明Ｒｆ０．９３处的组份能使水稻幼苗叶片的倾斜活性增加２５％，其放射性比强为７３．８ＧＢｑ／ｍｍｏｌ。Brassinosterone, a precursor inthe synthesis Of brassinolide, it exhibited more than 70% of the biological activity of brassinolide in rice test (Fig.2). Brassinthokime, a product of condensation of brassinosterone with Carboxymethoxylamine hydrochloride,showed 60% of that of brassinolide inthe same test system at 10 μmol/L concentration (Fig. 2 ). In cucumberhypocotyl test, the brassino-- oxime2 μmol/L ) promoted elongstion of cucumber hypocotyl by 20% as compered with control (Fig. 3 ). Thebrassino--chime was found to be comPetitive with brassinolide or brassinosterone (Fig. 4 ). Combination ofiodohistamine with brassino--oxime byChloramine--T method formed the 125Ilabelled compound which was used forcucumber and rice testS. The resultsshowed that the Rf 0. 93 fraction ofchromatography was effective in pro-moting both elongation and inclinationsignificantly (Fig. 5). So the Rf 0. 93was an effective one. Its specific radioactivity was 73. 8 GBq/mmol.

关 键 词：油菜素 内酯 甾酮 碘125 生物活性 

分 类 号：Q946.885[生物学—植物学]