绿豆上胚轴中油菜素甾酮的结合特性  

Preliminary Study of Brassinosterone Binding Sites from Mung Bean Epicotyls

作　　者：徐如涓 何宇炯[1] 王玉琴[1] 赵敏橘 

机构地区：[1]中国科学院上海植物生理研究所

出　　处：《植物生理学报（0257-4829)》1994年第3期298-302,共5页Acta Phytophysiologica Sinica

摘　　要：绿豆上胚轴中油菜素甾酮的结合特性徐如涓，何宇炯，王玉琴，赵敏橘（中国科学院上海植物生理研究所，上海２０００３２）关键词：油菜素甾酮，绿豆，上胚轴，结合位点近十年来的研究表明，由体类化合物广泛存在于植物体内，（Ｗｅｗｉｔｔ等１９８０，横田孝雄１９８７）...Different fractions of mung beau epicotyl extract weretcyted for brassinosterone binding.The 10 000 X gfraction showed stronger brassinosterone binding than other kactions(Table 1).The btassinosterone binding to 10 000Xg fraction was saturated after a 1h incubstion(Fig.1).The binding was temperature-dependent,showing a maximum at 4℃(Fig.2).The amount of brassinosterone bound Is dependent on pH,the Optimal pHbeing 7.5(Fig.3).TrvPsin treatment weakened the brassinosterone binding(Fig.4).This result indicates that proteins may be involved in the brassinosterone-binding site.Several analogues of brassinosterone could also bind to the braSSinosterolle-binding site to various extents(Table 2).

关 键 词：油菜素甾酮 绿豆 上胚轴 结合位点 

分 类 号：S522.01[农业科学—作物学]