绒毛石耳多糖UV-2和UV-3的分离、纯化及其性质  

ISOLATION, PURIFICATION AND CHARACTERIZATION OF POLYSACCHARIES UV-2 AND UV-3FROM UMBILICARIA VELLEA

作　　者：石皖阳[1] 商立坚 何伟[1] 文光裕[1] 胡昌序[1] 

机构地区：[1]中国科学院植物研究所

出　　处：《Acta Botanica Sinica》1994年第3期233-238,共6页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金

摘　　要：从绒毛石耳（Ｕｍｂｉｌｉｃａｒｉａ　　ｖｅｌｌｅａ（Ｌ）Ａｃｈ．）提取的水溶性粗多糖，经乙醇分级沉淀和ＤＥＡＥ一纤维素柱层析得到两个组分（ＵＶ－２和ＵＶ－３），经ｓｅｐｈａｒｏｓｅ６Ｂ柱层析和超速离心沉降鉴定为均一组分。通过糖组成分析、高碘酸盐氧化、Ｓｍｉｔｈ降解和甲基化分析证明，它们是带有部分乙酰基团的多分枝结构的杂多糖，ＵＶ－２的主链是由β（１→３）连接的葡萄糖和甘露糖残基构成，ＵＶ－３的主链是由β（１→３）（１→４）连接的葡萄糖残基构成。药理试验结果表明，ＵＶ－２具有增强免疫作用，能够明显促进小鼠腹腔巨噬细胞的吞噬功能，提高淋巴细胞的转化率。Two polysaccharides, coded UV-2 and UV-3, have been isolated by boiling water ex-traction from Umbilicaria vellea (L. ) Ach.. fractionally precipitated by 60% and 90%EtOH, and isolated through DEAE-cellulose column chromatography. They are homogeneous as judged by ultracentrifuge and filtration on sepharose 6B. By means of analysis ofsugar component, periodate oxidation, Smith degradation and methylation, UV-2 and UV-3were shown to be two highly brenched heteroglycans with few acetyl residues. The mainchain of UV-2 is composed of 0 (l-3) linked glucose and mannose residues, but the mainchain of UV-3 is composed of 0 (1-3) (1-4) linked glucosed residues. The results of pharmacological tests showed that activity inhibiting implanted S-180 in mice could not be foundin UV-2, but UV-2 was able to significantly stimulate phagocytosis of peritonealmacrophages and lymphocyte transformation.

关 键 词：绒毛石耳 多糖 免疫作用 分离 提纯 

分 类 号：R284.2[医药卫生—中药学]