河西走廊不同生态型芦苇核酸代谢季节动态研究  

SEASONAL CHANGES OF NUCLEIC ACID METABOLISM OF DIFFERENT ECOTYPES OF PHRAGMITES COMMUNIS IN THE CORRIDOR OF GANSU HEXI

作　　者：任东涛[1] 张承烈[1] 

机构地区：[1]兰州大学生物系,兰州730000

出　　处：《Acta Botanica Sinica》1994年第5期385-392,共8页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金

摘　　要：分布在甘肃河西走廊的４ 种生态型芦苇（Ｐｈｒａｇｍ ｉｔｅｓｃｏｍ ｍ ｕｎｉｓＴｒｉｎ．）的核酸代谢季节变化有差异。盐化草甸芦苇ＲＮＡ 含量持续增加，ＤＮＡ 含量相对稳定，其它３ 种生态型芦苇的ＲＮＡ 和ＤＮＡ 含量以５月份为最高。过渡带芦苇的ＲＮＡ 含量、沼泽芦苇及沙丘芦苇的ＤＮＡ含量９ 月份略有增高。盐化草甸芦苇与过渡带芦苇的ＤＮａｓｅ和ＲＮａｓｅ的活性７ 月份最高，沼泽芦苇与沙丘芦苇的ＤＮａｓｅ和ＲＮａｓｅ活性５—９ 月份呈增高趋势。盐化草甸芦苇的ＤＮＡ 和ＲＮＡ 合成活性不断升高，过渡带芦苇和沼泽芦苇的ＤＮＡ 和ＲＮＡ 合成活性及沙丘芦苇的ＲＮＡ 合成活性５—９月份均降低，仅沙丘芦苇的ＤＮＡ合成活性增强。ＲＮＡ 聚丙烯酰胺梯度凝胶电泳分析结果表明，４ 种生态型芦苇均含有２５Ｓ、２３Ｓ、１８Ｓ、１６Ｓ大分子量ｒＲＮＡ 和小分子量５．８Ｓ、５Ｓ、４．５ＳｒＲＮＡ 及４ＳｔＲＮＡ。大分子量ＲＮＡ 的含量高于小分子量ＲＮＡ 含量。不同生态型及同一生态型芦苇的不同发育时期，相同的ＲＮＡ 组分含量各不相同。且发育过程中２３Ｓ、１８Ｓ、１６ＳｒＲＮＡ 在不同月份发生不同程度的降解。由此，我们认为，核酸代谢的差异性是４Seasonal changes in metabolism of nucleic acids of different ecotype reeds growing in corridor of Gansu Hexi were investigated. The RNA content in salt meadow reeds rose continuously, where DNA remained constant.The contents of RNA and DNA in the other three ecotype reeds were highest in May. Content of RNA in salt meadow and dune transitional reeds rose a little in September, so did of swamp reeds and sand dune reeds. The activities of RNase and DNase of salt meadow reeds and salt meadow and dune transitional reeds were higher in July than other months; the activities of RNase and DNase of swamp reeds and sand dune reeds increased grandually from May to September. Synthetic rates of RNA and DNA of swamp reeds and salt meadow and dune transitional reeds, RNA of sand dune reeds decreased; but synthetic rate of DNA of sand dune reeds increased from May to September. Results from gradient PAGE assay of RNA from different ecotypes show that all four ecotype reeds contained large molecular weight rRNA of 25S,23S,18S,16S and small molecular weight rRNA of 5 8S, 5S, 4 5S and 4S tRNA. Contents of high molecular weight RNA were higher than low molecular weight RNA. Contents of same composition of RNA were different among four ecotype reeds and their growth respectively. The above results suggested that the difference of nucleic acids metabolism were the main cause of variation in the process from growth to senescence in the four ecotype reeds. Salt meadow reeds were well developed from May to September and the other three ecotype reeds developed well in May but went senescence to a different extents in July and September.

关 键 词：生态型 芦苇 核酸 代谢 衰老 

分 类 号：S564.201[农业科学—作物学]