用免疫亲和层析和ELISA测定百合花粉萌发过程中细胞分裂素含量的变化  

IMMUNOAFFINITY CHROMATOGRAPHIC AND ELISA DETERMINATION OF CYTOKININS IN GERMINA TING POLLENS IN LILIUM DAVIDII DACH.

作　　者：杨中汉[1] 陈良甫 曹宗巽[1] 

机构地区：[1]北京大学生物系,北京100871

出　　处：《Acta Botanica Sinica》1994年第6期430-436,共7页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家教委博士点资助

摘　　要：兰州百合（Ｌｉｌｉｕｍ ｄａｖｉｄｉｉＤａｃｈ．）花粉在ＰＥＧ－４００ 中萌发，用高度灵敏和特异的ｔ－ＺＲ－ＩｇＧ和ｉＰＡ－ＩｇＧ 免疫亲和层析分离纯化萌发花粉和萌发液中细胞分裂素，并用酶检疫连锁鉴定法（ＥＬＩＳＡ）测定Ｎ６－异戊烯腺嘌呤核苷（ｔ－ＺＲ）和异戊烯基腺苷（ｉＰＡ）含量。结果表明每克鲜重花粉含有３９ ｎｇ ｔ－ＺＲ和４８ ｎｇ ｉＰＡ。花粉水合后ｔ－ＺＲ 含量略有下降，而ｉＰＡ 含量明显增高，细胞分裂素总量几乎不变。水合过程中有细胞分裂素的消长，这种变化与花粉管的启动有关。在萌发的前３ 小时，花粉管生长速度最快，ｔ－ＺＲ和ｉＰＡ 在花粉管和萌发液中的含量也随着增加。Lilium davidii Duch.pollen was germinated in PEG 400.A rapid immunoaffinity chromatograpy system with high efficiency and specificity was adopted to isolate and purify trans zeatin riboside (t ZR) and isopentenyladenosine (ipA).Cytokinin determination was carried out by a highly sensitive enzyme linked immunosolvent assay (ELISA) for t ZR and iPA respectively.t ZR and iPA were present at the level of 10 -8 g/g fr.wt in Lilium davidii pollen.After hydration, t ZR content of pollen decreased slightly, while iPA increased remarkably, and the total amount of t ZR and iPA underwent almost no change.Pollen tubes grew fast in the first 3 h after germination, and the amount of t ZR and iPA also increased greatly both in pollen tubes and in germination medium.The increasing trend of t ZR and iPA and growth rate of pollen tubes was basically synchronous.

关 键 词：花粉 细胞分裂素 百合 ELISA 

分 类 号：Q949.718.2[生物学—植物学]